Culture Journal, Species: Chaetoceros muelleri

Author Message
KathyL
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Culture Journal, Species: Chaetoceros muelleri - Sunday, July 13, 2014 8:38 PM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Chaetoceros muelleri
Species description:  Golden brown phytoplankton, rectangular cells with antenna-like appendages
Culture source (link if possible):  Algagen via Seahorse Source
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  5/27/2014
Continuation Date:  8/27/14

Culturing Vessel Details
Salinity:  27 ppt
Temperature:    Ambient, about 75F
pH:  8

Vessel description:  One gallon plastic jar with lid. Hole in lid to accommodate rigid airline.
Lighting description:  Under-cabinet T5 fluorescent bulb, 18 watts.
Lighting cycle:  14 hours day, 10 hours dark
Aeration description:  moderate bubbling through rigid airline tubing

Methodologies
Split methodology: One to three dilution in newly sterile gallon jar, 3 ml Micro Algae Grow.

Culture medium description: 
Chlorinated, dechlorinated 27 ppt Instant Ocean saltwater, with 3ml Micro Algae Grow per gallon
Cell count:
 Unknown         

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Sunday, July 13, 2014 9:10 PM>
check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)
KathyL
  • Total Posts : 2639
  • Reward points : 1504
  • Joined: 6/6/2010
  • Location: St. Louis, MO, US
Re:Culture Journal, Species: Chaetoceros muelleri - Sunday, July 13, 2014 8:43 PM
When I purchased the culture it was lovely dark and did well on my shelf until I split it, and then it crashed.  I left the culture going, bubbling, and it appears to have recovered.  The pictures are of some cells that survived the crash, 400x via Celestron:

 



<message edited by KathyL on Sunday, July 13, 2014 8:55 PM>
check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)
KathyL
  • Total Posts : 2639
  • Reward points : 1504
  • Joined: 6/6/2010
  • Location: St. Louis, MO, US
Re:Culture Journal, Species: Chaetoceros muelleri - Thursday, July 24, 2014 12:36 AM
after initial "crash" it seems to be making a come back.  Split today for the second time.
<message edited by KathyL on Thursday, July 24, 2014 8:14 AM>
check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)
KathyL
  • Total Posts : 2639
  • Reward points : 1504
  • Joined: 6/6/2010
  • Location: St. Louis, MO, US
Re:Culture Journal, Species: Chaetoceros muelleri - Thursday, August 28, 2014 9:28 PM
The culture has been doing well, and I even have a serious bloom in my neon gobie BRT that showed up just as metamorphosis should have occurred. I have caught a glimpse of one meta goby, but I can't see any of the others, its that thick. I assume they are all OK.  
check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)