Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Chaetoceros muelleri
Species description: Golden brown phytoplankton, rectangular cells with antenna-like appendages Culture source (link if possible
): Algagen via Seahorse Source
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 5/27/2014
Continuation Date: 8/27/14
Culturing Vessel Details Salinity: 27 ppt
Temperature: Ambient, about 75F
pH: 8
Vessel description: One gallon plastic jar with lid. Hole in lid to accommodate rigid airline.
Lighting description: Under-cabinet T5 fluorescent bulb, 18 watts.
Lighting cycle: 14 hours day, 10 hours dark
Aeration description: moderate bubbling through rigid airline tubing
Methodologies Split methodology: One to three dilution in newly sterile gallon jar, 3 ml Micro Algae Grow.
Culture medium description: Chlorinated, dechlorinated 27 ppt Instant Ocean saltwater, with 3ml Micro Algae Grow per gallon
Cell count: Unknown
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Sunday, July 13, 2014 9:10 PM>
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