Culture Journal, Species: Nannochloropsis Sp.

General
Species:  Nannochloropsis Sp.
Species description:  Micro Algae
Culture source (link if possible):  Aquaculture Nursery Farms http://stores.aquaculturenurseryfarms.com/live-starter-phytoplankton-nannochloropsis-1-lt-bottle/
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  7/16/2014
Continuation Date:  

Culturing Vessel Details
Salinity:  1.021
Temperature: room temp 
pH: didn't measure (probably about 8.0) 

Vessel description:  2L Bottle
Lighting description:  48" 40W Florescent Shoplight with "Planted Aquarium" bulb
Lighting cycle:  12hr
Aeration description:  ridged airline tubing, rolling bubbles

Methodologies
Split methodology: When bottle gets dark green, it is split into two sterilized 2L bottles. The bottles are then topped off with new saltwater, and fertilized with 5-6mL of Miracle Grow 

Culture medium description: 
(this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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This is the bottle my starter came in

 
Starter after 4 days, right before split.

 
First Split

Splits on 7/22, 7/26, 7/29