Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tetraselmis
Species description: green large cell micro algae Culture source (link if possible
): Tami Weiss
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 7/20/14
Continuation Date: 7/1/2015
Culturing Vessel Details Salinity: 27 ppt
Temperature: 75F, ambient
pH: 8
Vessel description: one gallon square plastic wide mouth jar , hole in lid, rigid airline going to the bottom, connected to air source.
Lighting description: under cabinet t5 fluorescent tube suspended 2 inches from the jars
Lighting cycle: 14 hour day, 10 hours night
Aeration description: vigorous
Methodologies Split methodology: Split 1 to 3 in chlorinated, dechlorinated 27 ppt saltwater with 3 ml Micro Algae Grow (several years old)or Fritz's Guillard's formula per gallon, every 7 to 10 days
Culture medium description: chlorinated, dechlorinated 27 ppt saltwater with 3 ml Fritz's Guillards' formula at f strength, 3 ml per gallon jar
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: I added CO2 to the system on 8/9/14 to boost production.
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Friday, July 10, 2015 9:29 PM>
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