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Culture Journal, Species: Parvocalanus
Thursday, July 24, 2014 11:31 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Parvocalanus crassirostris Species description: calanoid copepod Culture source (link if possible): Starter culture obtained from Waldend, brought back from MBI conference If algae, CCMP # (Optional): http://ccmp.bigelow.edu/ Culture Establishment Date: 7/23/14 Continuation Date: 11/1/14 Culturing Vessel Details Salinity: 1.022 Temperature: Room temp, approx 72 degrees F at present pH: Approx 8.1 when newly mixed Vessel description: Clear plastic square containers, approx 1 liter volume Lighting description: Indirect 13w spiral cfl bulb (aimed at adjacent frag tank) Lighting cycle: 12 hrs on, 12 hrs off Aeration description: Very gentle bubble rate (1-2/second) rigid airline Methodologies Split methodology: thus far, when a culture appears well populated to the naked eye, half is harvested (fed to tank or used to start new culture). Existing culture is replenished with NSW Culture medium description: NSW prepared to SG of 1.022-1.023. Vessel is "fed" live t-iso when clear, just enough to barely tint the water. Cell count: (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by Lrood on Saturday, January 24, 2015 1:37 PM>
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Re:Culture Journal, Species: Parvocalanus
Thursday, July 24, 2014 11:33 AM
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Just starting this thread, will be updated once I get some more time. Thanks to Daniel (Waldend) for getting me the starter culture.
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Re:Culture Journal, Species: Parvocalanus
Thursday, July 31, 2014 8:19 PM
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Wow, are these little critters fast! I had a few naups under the scope today, and thought I'd take a pic to post, but they don't stay in the sites long! I suspect I will have to "sacrifice" a few on the slide with alcohol to get some photos. The cultures (I split my original yesterday, now have 2 running) seem to be growing great, with very light feedings of t-iso every couple of days or so.
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Re:Culture Journal, Species: Parvocalanus
Friday, August 1, 2014 11:27 AM
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They are definitely fast little buggers. Good to hear that your cultures are performing well. FYI, you can also put them down with household, distilled white vinegar. I use one drop per ml of sample (sample containing animals and saltwater).
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Re:Culture Journal, Species: Parvocalanus
Wednesday, August 6, 2014 5:18 PM
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I managed to get a pic through the microscope using my iPhone. Shows a couple of adults and some naups. I just split one of the cultures today and grabbed some in an eye dropper for the picture. So far the splits have been at about 6-7 days, and feedings with t-iso have been about every 2-3 days.
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Re:Culture Journal, Species: Parvocalanus
Thursday, October 2, 2014 3:38 PM
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Well, I thought I had lost my Parvo cultures from neglect. I haven't had any specific use for them, so they pretty much just sat in their little plastic bottles - 2 with airlines and very slow bubble rate, one with nothing. Whenever I had got around to putting in a little iso, I never noticed anything that looked alive. Last week, I decided to give each bottle a 50% water change, and added fresh t-iso, and all 3 bottles bloomed! I checked a sample with the scope to make sure, and indeed a mix of adults & naups. Amazing how resilient they are (luckily for me). Now to find a use for them!
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Re:Culture Journal, Species: Parvocalanus
Sunday, November 9, 2014 6:25 PM
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Re:Culture Journal, Species: Parvocalanus
Monday, December 15, 2014 8:29 AM
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Very cool. These are on my list but I need the T-iso to take first. Good job.
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Re:Culture Journal, Species: Parvocalanus
Monday, December 15, 2014 11:40 AM
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Did you take that picture with a microscope attachment with your iPhone or simply put the camera on the iPhone to the lense on the scope? Either way that's a pretty nice pic from a phone. I want to try it and maybe get a video the same way.
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Re:Culture Journal, Species: Parvocalanus
Monday, December 15, 2014 8:29 PM
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I made a DIY type of adapter that fits over an eyepiece on the scope. I still have to hold it there or else it will fall, but it gives me the correct standoff distance for images. There are commercial adapters out there, but I'm just being cheap ;-)
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Re:Culture Journal, Species: Parvocalanus
Saturday, January 24, 2015 1:28 PM
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Continuation reported on 11/1/14. Cultures still doing well, no changes in techniques.
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