Culture Journal, Species: Tetraselmis chuii

General
Species: Tetraselmis chuii
Species description: Green microalgae
Culture source (link if possible): MBI Conference (T. Weiss)
If algae, CCMP # (Optional):
[link=http://ccmp.bigelow.edu/]http://ccmp.bigelow.edu/[/link]
Culture Establishment Date:7/24/2014
Continuation Date: 12/31/2014

Culturing Vessel Details
Salinity: 1.024 currently, 1.020 initially. 
Temperature: Ambient Room Temp (70°F-78°F in summer, 60°F-70°F in winter)
pH:Not monitored

Vessel description: Current is 2L clear plastic container with screw on lid.  Hole drilled in lid to snugly fit a rigid airline.  Initial was 1 Quart Wide-mouth canning jars.  Plastic wrap cover held with rubberband around perimeter.  Rigid airline poked through wrap to feed air and a small puncture for air release.      
Lighting description: Single T8 bulb under-cabinet lighting fixture.
Lighting cycle: 18 on, 6 off.    Off is in the afternoon to reduce temp during the warmest part of day (summer and winter) to keep a more consistent temperature.  
Aeration description: Light boil to keep algae fully in suspension

Methodologies
Split methodology: Plan is to remove 75% every 7-10 days and add the remaining 25% to a new jar as an inoculation.   Actual ended up being minimal splits every 3-4 weeks.  Would add a second 20 drops of Micro Algae Grow if growth stalled.  Color would be extremely dark green at time of split.

Culture medium description: Current is 2l of ASW sterilized with chlorine at .8ml/2l , allowed to sit for 24hrs and then neutralized with equal parts sodium thiosulfate.  20 drops Micro Algae Grow added per 2l.  Initial was 1 litre of  artificial saltwater newly mixed and aged ~ 24hrs. Sterilized in microwave until the water boils in the jar.  Allowed to cool at room temp.  10 drops (~1/2ml) Micro Algae Grow added to the culture.  

Cell count: Not known
(if known)

Reference links:

Additional Information
(No Pictures or Videos in the Section Please)
Notes:



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Start on 7/24. 3rd from right (light green)



Same pic from 7/28. Darkened nicely.

Good color comparison to Iso. (A contaminated Iso is in background. I assume it's a Tet contamination since that is the only green I have).

Video of the culture for continuation.

Updated picture of the color.

Your culture looks great! I love tetraselmis! Who knew a plant could swim so well?

check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)

Thanks Kathy. This one does seem pretty bullet proof. I love getting it so dark you cannot see into it.

Now, if only Iso were so easy!