Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tetraselmis chuii
Species description: motile green algae
Culture source (link if possible
): Starter culture donated by Waldend, obtained from MBI conference
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 7/23/14
Continuation Date: 11/1/14
Culturing Vessel Details Salinity: 1.020
Temperature: room temp, approx 70-72 degrees F
pH: Not directly measured, but approx 8.1 when newly mixed
Vessel description: 1 quart plastic bottles
Lighting description: 13 w spiral cfl bulb
Lighting cycle: 16 hours on, 8 hours off
Aeration description: moderate bubble rate with rigid airline
Methodologies Split methodology: Split as cultures mature. At 7-8 days, when culture is dark green, new culture bottle is inoculated with approx 25% of mature bottle (remainder used for feed).
Culture medium description: 20 drops Guillard's f/2 per 1 quart of NSW mixed at SG 1.020. Sterilized by (1) microwaving to 180 degrees F and allowing to cool to room temp, or by (2) Chlorox technique (1 ml standard Chlorox per gallon of media; dechlorinated with 0.4ml of Drs. Foster & Smith Chlorine Neutralizer per 1 quart bottle at least 15 minutes before inoculation).
Cell count: not presently measured
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by Lrood on Saturday, January 24, 2015 1:36 PM>