Tigriopis californicus eggs and nauplii

Hey all,
 
Does anyone know how big the eggs of T. californus are?  How about newly hatched nauplii?  Are the eggs neutrally buoyant or sinking?  I can't seem to find this information anywhere.
 
I've been wondering if I'm throwing out naups and maybe even eggs when I'm sieving through 180 microns mesh.  I should check my sieved water too...

Don't let fear and common sense stop you! =]

I don't have a source for the data but I would say yes you are losing the eggs. I will have to check my screen size but I believe mine is 150 and I can plainly see that I have knocked the eggs off the females and lost them after sieving.

Nearly positive the eggs do not hatch once knocked off, so loss of eggs isn't a big deal (once knocked off)... but yeah, you are loosing naups.  Use your rotifer sieve to get the naups.

Ok thanks Gresham!  I will get another rotifer sieve...rotifers hav wings and they don't need to be in my Tig cultures.    I think I'm going to start sieving the Tigs through bigger micron too.  Maybe find something that the males and females without eggs can go through, but the females with eggs will stay on top so I can put them back. 

Don't let fear and common sense stop you! =]

When I stopped aerating my Tigger culture, the rot problem went away.    My Tiggers do great in stagnant water.
 
I have the opposite problem though, Tiggers in my rotifer culture!  I'm not too worried about it, but screening them with 120 micron doesn't make them go away... some naups slip through and a couple weeks later they're back again!  I guess if I were determined, screening them daily for a week or two would probably do it.

So far, my cultures are mono-cultures.  Hopefully they stay that way.   I don't aerate my Tigs either.  I did have rotifers in my Tig cultures a couple years ago, and the population of rotifers crashed the cultures, so I had to restart.

Don't let fear and common sense stop you! =]

When I was doing small cultures, I had to aerate when I had denser cultures.  If not, I'd see piles of dead ones in the corners.

 
Hmm, interesting.  I experimented recently with adding very light aeration to a couple vats and found them to be negatively affected.

Don't let fear and common sense stop you! =]

was the airstone resting on anything?  That's counter productive I've found.

I had this same experience, too... the stagnant cultures seemed to do better.  I was using PhytoFeast last time I tried it, so I don't know if that played into it somehow.

 
No stone, just open end rigid airline tubing.  About 2-3 bubbles per second.

Don't let fear and common sense stop you! =]

resting on bottom?