Culture Journal, Species: L Strain Rotifer (Brachionus plicatilis)

General
Species:  Brachionus plicatilis
Species description:  Large Strain Rotifer commonly available
Culture source (link if possible): http://florida-aqua-farms.com/
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  9/ 24/ 2014
Continuation Date: 

Culturing Vessel Details
Salinity:   1.023  varies
Temperature:    room temperature
pH:   8  varies

Vessel description:  2.5 gallon drinking water container, 65 gallon waste tank, backup cup
Lighting description:   ambient from algae fluorescent strips
Lighting cycle:  14-16 hours a day
Aeration description:  light bubbles, heavy aeration, none (in order of vessel listed above)

Methodologies
Split methodology:  right now not splitting, harvesting and feeding excess to tank.

Culture medium description:  Main culture, cup backup is fed RGComplete 2x daily.  65 gallon is a live nanno co-culture (newly started)

Cell count: Not currently known.

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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Note the 65 gallon tank is my attempt to establish a low density self perpetuating culture.  If I can keep the density low then the nanno should be able to keep up production and feed the culture.  Eventually this will become my model for a larva rearing tank with constant baby rotifers available with the nanno providing nutrient uptake (hopefully). I have heard it can't be done but that won't stop me from trying.  I would eventually also do additions of other algae like T-Iso when I get larva to improve the nutritional profile. Even though the T-Iso won't co-culture with it in the water the rotifers would still uptake the algae.

Picture of the coffee filter I use