Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Euterpina acutifrons
Species description: Harpacticoida
Culture source (link if possible
): (MBI Conference - T. Weiss)
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 7/24/14
Continuation Date: 2/8/15
Culturing Vessel Details Salinity: 1.024
Temperature: Ambient Room Temp (70°F-78°F in summer, 60°F-70°F in winter)
pH: Not tested
Vessel description: 2L clear plastic container with screw on lid. Hole drilled in lid to snugly fit a rigid airline
Lighting description: Single T8 bulb under-cabinet lighting fixture.
Lighting cycle: Semi-direct light from phyto culture fixture. 18 on, 6 off. Off is in the afternoon to reduce temp during the warmest part of day (summer and winter) to keep a more consistent temperature.
Aeration description: 1 bubble per second max. Just enough to move the water to keep the copepod in suspension. Copepods can be seen moving against the flow but upon resting the move with it.
Methodologies Split methodology: Culture is poured through a 23micron sieve to remove all copepods. They are then backwashed into a container with fresh ASW.
Culture medium description: 2l of ASW sterilized with chlorine at .8ml/2l , allowed to sit for 24hrs and then neutralized with equal parts sodium thiosulfate. Enough Tet algae is added once per week to tint the water a light green.
Cell count: Not counted
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: I have not really cultured these for use so everything was minimalized after the initial ramp in order to simply maintain 2 cultures.
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.