Culture Journal, Species: Brachionus Plicatilis

General
Species:  Brachionus Plicatilis
Species description:  L-type Rotifer
Culture source (link if possible):  Tal Sweet
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  11/22/14
Continuation Date: 

Culturing Vessel Details
Salinity:  1.020
Temperature:    ambient room tempeture around 72 degrees F
pH:  Unknown

Vessel description: 5 gallon bucket 
Lighting description:  ambient lighting from breeder tanks.
Lighting cycle:  14hrs on 10hrs off.
Aeration description:  constant aeration

Methodologies
Split methodology: my buckets are filled with 4 gallons of water. I scoop out and sieve a gallon every 2 weeks and feed it to my reef, And take what I need for my hatchlings. I clean out the bucket once a month. I keep 2 gallons with rotifers and add 2 fresh gallons with  a 1.020 salinity.

Culture medium description: 
I split 1 liter of RG evenly into 24 cup cake tins. I mix one frozen tin in a 2 liter of RO water and feed 40ml of deluted rotigrow twice a day.

Cell count: 
 unknown

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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Here is a video.
https://youtu.be/dEHM-GwHzQo

Thanks Ray
 
A very good guide.

Jake
We are all in this together

No problem Jake.