Culture Journal, Species: [isochrysis galbana]

General
Species:  isochrysis galbana
Species description:  motile brown algae
Culture source (link if possible): 
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  July 30, 2014
Continuation Date:  still going
http://i893.photobucket.com/albums/ac132/aquatictec_photos/MBI/isoculture20150524-2.jpg
Culturing Vessel Details
Salinity:  30.5ppt
Temperature:   72-78  
pH:  never measured

Vessel description:  6 gallon clear plastic carboys
 

Lighting description:  72 watt led light bar 2 per 4' or 4 bottles
Lighting cycle:  24/7 continuous
Aeration description: 
filtered outside air vigorous aeration with rigid airline tubing and central blower
Methodologies
Split methodology:  once per week 50 percent

Culture medium description:  
sterilized salt water with 2.5 ml per gallon of Guillards F/2 formulation per gallon

Cell count: 
 not performed
dark brown color when split
Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes:  we use fresh saltwater made with red sea salt.  We keep 7 cultures going.  We sometimes loose some of them but we just split the remaining ones


You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.