Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: tetraselmis suecica
Species description: single cell motile green algae Culture source (link if possible
): http://www.algagen.com/?p=35 If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 06/30/2014
Continuation Date: still going 06/13/2015
Culturing Vessel Details Salinity: 30.5ppt
Temperature: 72-78 F
pH: not measured
Vessel description: 6.5 gallon clear plastic carboys
Lighting description: white/blue /red 3 watt LEDs various mixtures 2 -72 watt strips per 4' of culture row (equates to 4 carboys) color temperature does not seem to make a dfifference
Lighting cycle: 24/7
Aeration description: central blower with filtered outside air
vigorous aeration with single 3/16" rigid tubing per carboy
Methodologies we have 6 cultures going:
In the picture below the 2 cultures on the left are tetraselmis 5 days old
http://i893.photobucket.com/albums/ac132/aquatictec_photos/tc3.jpg The picture below is a close up of a 5 day old culture
http://i893.photobucket.com/albums/ac132/aquatictec_photos/tc2.jpg In the picture below the 4 cultures on the left are tetraselmis 5 days old
http://i893.photobucket.com/albums/ac132/aquatictec_photos/tc1.jpg Split methodology: prepare sterile carboy stopper and rigid airline
pour of top 1/2 of culture into new carboy add sterilized salt water to fill
add 4 ml Guillards F/2 formulation
Culture medium description: sterilized salt water with 2.5 ml per gallon of Guillards F/2 formulation per gallon
Cell count: not performed
dark green color before splits
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.