Culture Journal, Species: [Brachionus plicatilis ]

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aquatictec
  • Total Posts : 8
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  • Joined: 5/8/2014
  • Location: Columbia Station, OH, US
Culture Journal, Species: [Brachionus plicatilis ] - Saturday, June 13, 2015 4:19 PM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Rotifers L Type - Brachionus plicatilis
Species description:  160-360 micrometers
Culture source (link if possible):  http://reedmariculture.com/support_rotifers_feeding.php
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  Over 10 years ago
Continuation Date:  still going 06/13/2015

Culturing Vessel Details
Salinity:  varies
Temperature:    varies 70-84
pH:  8-8.2
picture of our 2 rotifer culture tanks:
http://i893.photobucket.com/albums/ac132/aquatictec_photos/rot1.jpg
Vessel description:  10 gallon tanks
Lighting description:  varies - fluorescent tubes overhead
Lighting cycle:  24/7
Aeration description:  rigid airline 2 tubes per tank

Methodologies
Split methodology: weekly on Monday harvest 8 gallons take 10 gallon tank down to 2 gallons
set up clean tank
on Monday, Wednesday and Friday we add 2.5 gallons of saltwater per culture
Culture medium description:  
fresh saltwater not sterilized made from Red Sea salt
We feed daily with cultured phytoplankton nano or iso  

Cell count: varies - as low as 40 and up to 1000 or more /ml

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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<message edited by aquatictec on Saturday, June 13, 2015 5:11 PM>

Jake
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  • Location: Mikado, MI, US
Re:Culture Journal, Species: [Brachionus plicatilis ] - Sunday, June 14, 2015 8:45 AM
Congratulations for the age of the culture !  Are there ciliates and others in it yet?  I have been thinking of the same with two five gallon tanks, or 3 gallon Walmart water jugs with spigots and large side lid.
Jake
We are all in this together

aquatictec
  • Total Posts : 8
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  • Joined: 5/8/2014
  • Location: Columbia Station, OH, US
Re:Culture Journal, Species: [Brachionus plicatilis ] - Sunday, June 14, 2015 1:00 PM
we use a cleaned tank each week and take the cultures down to just a few gallons
sometimes brine shrimp get in we just strain them out