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Culture Journal, Species: Rhodomonas lens
Saturday, June 20, 2015 1:20 PM
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Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Rhodomonas lens Species description: RED microalgae, a phytoplankton Culture source (link if possible ): Tami Weiss , a kind gift If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 4/9/2015 Continuation Date: 7/12/2015 Culturing Vessel Details Salinity: 27-30 ppt Temperature: ambient, approximately 75F pH: 8
Vessel description: 1 gallon plastic wide mouth jars with hole in cap, rigid airline connected to output of linear piston pump to provide air. Lighting description: single undercabinet fluorescent light mounted on side of vessel agout 3 inches away Lighting cycle: 14 hours daylight, 10 hours night Aeration description: vigorous, as if at a hard boil Methodologies Split methodology: To a jar and lid setup, chlorinated and dechlorinated, including the 2/3 (roughly) gallon culture water, 3 ml Guillards (f), add 1/3 gallon darkest rhodamonas culture I have. Culture medium description: 3 ml Guillard's to a gallon of culture, 27-30ppt saltwater, chlorinated and dechlorinated previously to "sterilize". Cell count: unknown Reference links: http://www.ipg.pt/udi/files/Reference%205.pdf Additional Information (No Pictures or Videos in the Section Please) Notes: Daniel Walden asked about chloramines caused by the addition of bleach to the culture water: I think that in order for chloramines to be present, one would have to have ammonia mixed with chlorine-not sure. My saltwater is prepared with water that has gone through a chloramine removal filter, and while I have not tested for chloramines, I do store the water in a tub that certainly has accumulated enough bacteria to inactivate any ammonia or chloramines that may have bypassed the filter. The water tests as having no ammonia. I then add chlorine to water and gallon jar so that everything is sterilized at the same time, and then dechlorinated. Then I test for the presence of chlorine, just to make sure. I do all this before I add the fertilizer, so I don't know how ammonia would get in there, so all this is to explain that I am unconcerned about chloramines in my phyto cultures. You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Sunday, July 12, 2015 6:34 PM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodomonas lens
Saturday, June 20, 2015 6:10 PM
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Left to right, under done tetraselmis, overdone isochrisis, and just right rhodomonas:  love, Goldilocks.
<message edited by KathyL on Tuesday, June 23, 2015 8:23 AM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodomonas lens
Saturday, June 20, 2015 6:10 PM
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duplicate post….
<message edited by KathyL on Tuesday, June 23, 2015 8:26 AM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodomonas lens
Monday, June 22, 2015 12:20 AM
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I took this charming note's picture as I unpacked the box from Tami in April: 
<message edited by KathyL on Tuesday, June 23, 2015 8:27 AM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Monday, June 22, 2015 2:22 PM
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As the sign says,it is RHODO,not RHODA. 
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Re:Culture Journal, Species: Rhodamonas lens
Tuesday, June 23, 2015 8:29 AM
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check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Sunday, July 12, 2015 6:15 PM
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Time for the continuation report: pix to follow
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Sunday, July 12, 2015 6:28 PM
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It's the red one on the right: 
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Thursday, July 16, 2015 10:41 PM
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Seems to be going into the brown phase that Tami was talking about.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Friday, July 17, 2015 2:15 AM
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What's this about a brown phase?
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Re:Culture Journal, Species: Rhodamonas lens
Friday, July 17, 2015 7:00 AM
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Tami Weiss, who gave me the starter, says that hers sometimes goes brown and declines, and then comes back red again if you just let it stay on the shelf. I'm hoping I have the same experience, because it is not doing so well at the moment.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Thursday, July 30, 2015 8:41 AM
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Further consultation from Tami says that more fertilizer is required. Before MbI, I split the struggling culture, adding f to both the new and the struggling one. The new one turned faintly, but definitely, pink immediately. The older culture crashed. The new one is now, days later, getting more reddish and is growing.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Rhodamonas lens
Thursday, September 17, 2015 2:58 AM
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Hows this culture doing Kathy? This definitely is a tricky one. What did you ended up raising the nutrients to? 4ml/gallon?
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Re:Culture Journal, Species: Rhodamonas lens
Thursday, September 17, 2015 8:57 AM
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No, it bounced back and is still going. Recently I sent some back to Tami, as hers crashed. It's good to have a fishy friend.
New thing: I get this green clumpy algae mixed in with the culture--happens with the Isochrysis I have as well. I filter the culture (non-sterile) through a 30 micron sieve and the green abates for a while and the red rebounds. Not sure how sustainable this is, but I still have a red phyto, assuming it's still Rhodomonas, and a brown culture, assumed Isochrysis. I haven't increased the fertilizer concentration.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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