Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Rhodomonas lens
Species description: RED microalgae, a phytoplankton Culture source (link if possible
): Tami Weiss , a kind gift
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 4/9/2015
Continuation Date: 7/12/2015
Culturing Vessel Details Salinity: 27-30 ppt
Temperature: ambient, approximately 75F
pH: 8
Vessel description: 1 gallon plastic wide mouth jars with hole in cap, rigid airline connected to output of linear piston pump to provide air.
Lighting description: single undercabinet fluorescent light mounted on side of vessel agout 3 inches away
Lighting cycle: 14 hours daylight, 10 hours night
Aeration description: vigorous, as if at a hard boil
Methodologies Split methodology: To a jar and lid setup, chlorinated and dechlorinated, including the 2/3 (roughly) gallon culture water, 3 ml Guillards (f), add 1/3 gallon darkest rhodamonas culture I have.
Culture medium description: 3 ml Guillard's to a gallon of culture, 27-30ppt saltwater, chlorinated and dechlorinated previously to "sterilize".
Cell count: unknown
Reference links: http://www.ipg.pt/udi/files/Reference%205.pdf Additional Information (No Pictures or Videos in the Section Please) Notes: Daniel Walden asked about chloramines caused by the addition of bleach to the culture water:
I think that in order for chloramines to be present, one would have to have ammonia mixed with chlorine-not sure. My saltwater is prepared with water that has gone through a chloramine removal filter, and while I have not tested for chloramines, I do store the water in a tub that certainly has accumulated enough bacteria to inactivate any ammonia or chloramines that may have bypassed the filter. The water tests as having no ammonia. I then add chlorine to water and gallon jar so that everything is sterilized at the same time, and then dechlorinated. Then I test for the presence of chlorine, just to make sure. I do all this before I add the fertilizer, so I don't know how ammonia would get in there, so all this is to explain that I am unconcerned about chloramines in my phyto cultures.
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<message edited by KathyL on Sunday, July 12, 2015 6:34 PM>