Breeding Journal, Species: [Amphiprion Ocellaris (Frostbite X WW]

General
Species:  Amphiprion Ocellaris
Social Structure:  Pair
Size of Individuals:  Female 3.5" Male 2.25"
Age of Individuals:  Both 2.5 years
Date added to Tank:  Oct 7 2014

Broodstock Tank Details
Size of Tank:  5 gallon (in 50gal system)
Substrate Details:  None
Filtration Details:  sump with skimmer and LR
Water Changes:  20% weekly
Water Temperature:  81.9~83.2 F
Lighting:  small CFL 5000K
Lighting Cycle:  13on/11off
Other Tank Inhabitants:  none

Broodstock Feeding Details
Food Types:  NLS/ homemade blend
Feeding Schedule:  3 times daily (10am/1:30pm/5:30pm)

Spawning Details
Date of First Spawn:  June 20 2015
Spawn Time of Day:  5PM
Dates of Consecutive Spawns:  every 9~10 days
Courtship Details:  male chase female, and bitting females belly. both clean the pot together for 2 days.
Egg Size:  1mm
Egg Color:  orange
Egg Count:  200~300

Hatch Details
Hatch Date:  June 27 2015
Hatch Time of Day:  8pm after lights turn off
# Days after Spawn:  7
Larvae Description:   "normal" same as other clown fry. silver color about 3 mm in size
Consecutive Hatch Dates:    1 : all hatched on same night


Larval Tank Details
Temperature:  79.7~80.6
Size of Larval Tank:  10gal with 5gal of newly mixed saltwater
Substrate Details:  none
Other Tank Decor:  air stone
Filtration Details:  none
Lighting:  3W led
Lighting Cycle:  24 on
Water Changes:  none for the first 5 days

Larval Feeding Details
Food Types:  Rotifers for the hatching nights. mixed BBS with rotifers on day 2,3, stop rotifer on day 4.
Feeding Schedule:  once a day

Metamorphosis/Settlement
Date of Settlement Start:  July 4 2015
Days after Hatch:  7
Date of Settlement End:  July 7 2015
Description of Fry:  most have first stripe. some with wider first stripe( probably going to be WW)

Grow-Out Tank Details
Temperature:  79.7~80.6
Size of Grow-Out Tank:  10
Substrate Details:  none
Other Tank Decor:  air stone
Filtration Details:  none
Lighting:  small CFL
Lighting Cycle: 13on 11off               
Water Changes:  80% every 4 days
Size at Transfer: 1"               
Age at Transfer:  30~35 days i move them into grow out with filteration.

Grow-Out Feeding Details
Food Types:  TDO
Feeding Schedule:  5 times daily

Additional Information
collected my first batch 2 month ago. i raised them and make sure i succeed before i post here.
(No Pictures or Videos in the Section Please)
Miscellaneous Information: 



You will be required to provide photographic or video evidence in this thread of each event submitted for the MBI Program.
If your thread does not contain these photos the MBI Committee will not be able to approve your reports. PHOTOS AND VIDEO S MUST BE PLACED IN ADDITIONAL POSTS, NEVER IN THE FIRST POST IN A JOURNAL.

looks like for over a year's... i still haven't figure out how to upload pics...
 

finally....
pair before laying eggs.
 
 

DAY 1, with "green water"

day 15, some of them are almost white!

45 days old. finally be able to see the spot on them

Those little guys look great!

Thats a great looking pair and babies! Whats the mix of babies you ended up with? Wyoming white, davinci, normal…?

Yes, can we get more baby pictures please?

thanks everyone...the first batch is reaching salable size now!
here is a few more pics.

another thing i want to mention.  According to Genetics Calculator, reef builders article, and my own experience, i should get 1/4 davinci, 1/4WW, 1/4 flurry and 1/4 frostbite. however with the result of the first few batches of the babies, im getting roughly 30~40% ocellaris. only few Low grade davincis. (less than 3 every 100fish). im guessing, all those ocellaris have Davinci gene, but just not showing up on the markings. ??? any thoughts?

That's strange I would not have predicted any normal striped ocellaris. Nice looking fish in the picture!

Talking with Soren from Sea and Reef, I was under the impression that the diVinci gene separate from WW or snowflake genes.??

i think ww and davinci have same gene... it's just, they need multi generations to get those fancier davinci..

Davinci and Wyoming white are the same gene. If the fish gets a single dose you get a davinci and if it gets a double dose it's a Wyoming white. A davinci crossed with a davinci will give you approximately 25% normal ocellaris, 50% davinci, and 25% Wyoming white.

Thanks for the detail.
problem is, my 25% Davinci end up being all regular ocellaris~~~
 

Seems to me, if you cross a single allele mutation with a double allele mutation you should get half of the progeny with a single allele, and half double allele.  There should be no normal. That's assuming that in the double allele the mutations are on each of the chromatids of the same chromosome.  If both mutations are on the same chromatid, then half of the progeny should be normal, 25% single mutation, 25% double. Not sure.

check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)