MBI Event Reporting Form

GENERAL
Name:	Gina Hayward ( GinaReef ) Site: Marine Breeding Initiative (MBI)
Species:	Nannochloropsis Sp. ( Class: FC1 , Points: 5 )
Establishment Date:	12/17/2012
Culture Source:	Reef Crew, Barrie, Ontario, Canada ( Bionomic Solutions)
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=78913

Culturing Vessel Details
Temperature:	68* ° F
PH:	not tested
Specific Gravity:	approx 1.015
Vessel Description:	2 liter soda bottles
Lighting:	2 x 13 watt compact fluorescent (powersaver 60 watt incandescent equivalent)
Lighting Schedule:	16 hr on /8 off

Methodologies
Feeding Tactics/Medium:	f/2 gulliard's in ASW 1/4 of bottle fed to rotifers for 2 days. Bottle topped up with Gulliard's f/2 and ASW and allowed to darken for 5-6 days.
Split Cycle:	5-6 days 3 x 2 liter bottles 1/4 bottle used daily to feed rotifer culture
Split Yield:	unknown
Number of Cultures:	3
Number of Splits:	unknown
Density:	unknown
Harvest Methology:	1/4 bottle used to feed rotifers daily
Adjustments Made:	none at this time

Other Details
Other Information: