MBI Event Reporting Form

GENERAL
Name:	Heather Mutschler ( altolamprologus ) Site: Marine Breeding Initiative (MBI)
Species:	Tigriopus californicus ( Class: FC1 , Points: 5 )
Establishment Date:	07/14/2011
Culture Source:	reef nutrition
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=81975

Culturing Vessel Details
Temperature:	62-80, usually around 68 ° F
PH:	not tested
Specific Gravity:	1.025-1.050, usually 1.030-1.035
Vessel Description:	5 gallon buckets and 2 liter soda bottles
Lighting:	A single T8 bulb
Lighting Schedule:	~8 hours a day

Methodologies
Feeding Tactics/Medium:	Phyto-feast live added about once a week
Split Cycle:	50% split 2-8 times per month
Split Yield:	A couple thousand for the buckets, a few hundred for the bottles
Number of Cultures:	4
Number of Splits:	Multiple dozens per container
Density:	~100-300 per liter
Harvest Methology:	Pour water through a sieve to collect the pods and replace the water with filtered and sterilized natural seawater
Adjustments Made:	The cultures were originally started in mason jars, then moved to 2 liter soda bottles, then after a couple months split to 5 gallon buckets. They used to be aerated, but have been without any aeration for about a year.

Other Details
Other Information:	One of the 4 cultures is co-cultured with brine shrimp. They seem to do better than the ones in single species cultures.