MBI Event Reporting Form

GENERAL
Name:	Nathan Belk ( nrbelk ) Site: Marine Breeding Initiative (MBI)
Species:	Tisbe biminiensis ( Class: FC1 , Points: 5 )
Establishment Date:	03/04/2013
Culture Source:	LiveAquaria - http://www.liveaquaria.com/product/prod_display.cfm?c=747+3137+3058&pcatid=3058&siteid=20&&topcatid=2190
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=82034

Culturing Vessel Details
Temperature:	68-70 ° F
PH:	Not Measured
Specific Gravity:	1.022
Vessel Description:	Inverted 5 Gallon carboy water jug. Filter sponges are being used to provide extra surface area.
Lighting:	Ambient Room light
Lighting Schedule:	N/A

Methodologies
Feeding Tactics/Medium:	Feed as often as needed to keep the water slightly tinted. Feed consists of a mix of Nanno, Iso, and Tetra. Medium was contaminated with rotifers and no attempts have been made to separate them from each other.
Split Cycle:	Typically once a week.
Split Yield:	Not sure what the actual numbers are but I estimate that I can get at least 600 individuals from one sponge.
Number of Cultures:	Currently only 1. The second culture vessel is being prepared
Number of Splits:	4
Density:	unknown but 1 per 5 ml would be my guesstimate.
Harvest Methology:	Squeeze/shake/wash out sponge over 150um sieve
Adjustments Made:	I have allowed the rotifers that contaminated the culture to remain as I believe they are helping me keep everything more stable. They also help me reduce the problems if I overfeed. Or so I believe.

Other Details
Other Information:	Am considering switching the sponges out for bathroom loofas for increased surface area, expense, and ease of use.