MBI Event Reporting Form

GENERAL
Name:	Shannon Pechauer ( shannpeach ) Site: Marine Breeding Initiative (MBI)
Species:	Parvocalanus crassirostris ( Class: FC2 , Points: 10 )
Establishment Date:	05/07/2014
Culture Source:	http://apbreed.com/product_parvocalanus_crassirostris.php
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=89323

Culturing Vessel Details
Temperature:	Room temperature, roughly 75 lately ° F
PH:	Not measured
Specific Gravity:	~1.023
Vessel Description:	1 and 0.5 gallon clear cereal containers, 5.5 gallon aquarium
Lighting:	Fluorescent strip light
Lighting Schedule:	~6AM to 9PM

Methodologies
Feeding Tactics/Medium:	I feed Isochrysis enough to just barely tint the water (or sometimes not even tint the water)
Split Cycle:	No set cycle at the moment. I split a culture in half when it looks to start to bloom and create a new culture to scale up.
Split Yield:	Not counted at this time since still scaling up and learning to culture them in high densities.
Number of Cultures:	4
Number of Splits:	~3
Density:	~1 per mL, maybe more, maybe less
Harvest Methology:	Not harvested yet
Adjustments Made:	I stopped using sterilized/neutralized water and now just use water sieved through 27 micron mesh.

Other Details
Other Information:	This is probably the 5th time I tried this species...and I finally seem to be getting the hang of it.