MBI Event Reporting Form

GENERAL
Name:	Ray Holler ( ray2111 ) Site: Marine Breeding Initiative (MBI)
Species:	Brachionus plicatilis ( Class: FC1 , Points: 5 )
Establishment Date:	11/22/2014
Culture Source:	Tal Sweet
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=94433

Culturing Vessel Details
Temperature:	room tempeture around 72 degrees ° F
PH:	unknown
Specific Gravity:	1.020
Vessel Description:	2 five gallon buckets.
Lighting:	ambient light from breeder tanks.
Lighting Schedule:	14hrs on 10hrs off.

Methodologies
Feeding Tactics/Medium:	I dilute the RG. I evenly split a 1 liter bag of RG into 24 cup cake tins, then mix 1 frozen cup cake tin into a 2 liter of RO water and feed 40 ml of deluted rotigrow twice a day.
Split Cycle:	I pour 2 gallons into a clean bucket and replace the other 2 gallons with new water.
Split Yield:	unknown
Number of Cultures:	2
Number of Splits:	several dating back to 11/22/2014
Density:	unknown
Harvest Methology:	I use a 120um sieve to catch debris and a 53um sieve to catch the rotifers.
Adjustments Made:	none yet.

Other Details
Other Information:	These mutiply very fast. I have not had any crash yet. very easy to keep