Methodologies |
Feeding Tactics/Medium: |
F2 formula 4ml per 2.5 gallons of new water added to 2.5 gallons of culture
|
Split Cycle: |
weekly |
Split Yield: |
50 percent |
Number of Cultures: |
7+ |
Number of Splits: |
approximately 40 |
Density: |
no cell counts dark coffee color before splitting
|
Harvest Methology: |
pour out top half to start new culture
use bottom half
bottles, caps and airline tubes sterilized with bleach and or acid |
Adjustments Made: |
switched form glass to plastic carboys
they were easier to clean and far less dangerous |