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Re:Phytoplankton Culture
Saturday, September 7, 2013 9:35 AM
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Given Jim's reference above about nannochloropsis having no distinct morphological characteristics, I just curious how definitive ID is made by the experts? Does that just refer to the various species of Nannochloropsis being difficult to tell apart, or all green non motile algaes? That said, I'm just a hobbyist and it does really matter to me as long as my cultures are healthy!!
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Re:Phytoplankton Culture
Saturday, September 7, 2013 1:25 PM
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 Originally Posted by jazzybio13
Jim- you are right in the sense that you can't scope ID down to species but in a very general sense you can look into a scope and darn well see if things are going solid or not. A good clean Nanno culture will be just as you said green, round, and non motile. But in my experience (and humble opinion) if you are visually inspecting a culture **algae, pods, or whatever it may be** you will tend to catch things. A quick and easy look in a scope would show multiple 'shapes'-round, crescent, and or rods, etc. and or if the culture was motile or not. This should be general enough.
All in all, no- you can't get a positive ID, but you can get a good idea of what condition your culture is in. I have had a healthy green nanno culture with nice perfectly round non motile cells and I have had a green 'nanno' culture with all sorts of contamination. And I can tell you that contaminated one will crash every time- much in the same fashion rj's describing.
Well put! This is exactly the kind of response I was hoping to elicit. Thanks!
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Re:Phytoplankton Culture
Saturday, September 7, 2013 5:31 PM
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oh jim.. you tease! I know full well you can't get a good ID on 'em... but if you wanted me to write it out and clarify... will do!  (hey, maybe you should throw that tidbit in planktopedia.org...) or at least some GOOD pics on the 'learn' pages for each species. I know you have links to ccmp which has photo's but would be nice to see it all in one place.  wink wink! Lrood- it says further down in Jims source : ...."The characterisation is mostly done by rbcL gene and 18S rDNA sequence analysis"... it also says later on that there nanno lacks the chlorophyll b and c, as one of it's distinguishing characteristics. But all in all I'd say basically it's a bit more than we need to mess with in our hobby! I'd tend to agree with you that as a hobbyist as long as our cultures are healthy... we are happy.
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Re:Phytoplankton Culture
Saturday, May 24, 2014 4:15 PM
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Question for you phytoplankton gurus: How much sodium metasilicate is needed to grow diatoms? I'm either having a mental blank on how to google for this info, or its just not out there. I will place an order from FAF.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Phytoplankton Culture
Saturday, May 24, 2014 4:23 PM
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Good question Kathy, I'm in a similar position. I bought a bunch of mass packs from FAF and need to add silicate for the diatoms and I also await an answer. I believe that FAF will sell the silicate separately to help us out.
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Re:Phytoplankton Culture
Saturday, May 24, 2014 4:29 PM
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I have another question for the phyto growers out there. I have 275 gallon totes in a greenhouse under sunlight. Would nanno and Iso grow OK in totes? For now, I'm going to leave the 6" circular tops sealed to help prevent contamination, especially on Iso. But I wonder if anyone else has had experience with these tanks. I've seen some pics of people that grow phyto in them outdoors for algae biofuel but it seems that once the phyto starts plating on the sides that would be hard to clean off. Even a chlorine soak after a crash would kill the plated algae but not necessarily remove it from the sides of the container, there would need to be a physical scrubbing which would not be possible unless the tote had an open top. Thanks in advance.
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Re:Phytoplankton Culture
Saturday, June 7, 2014 11:37 AM
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Has anyone grown phyto in a 275 gallon tote?
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