Change Page: < 1234 | Showing page 4 of 4, messages 61 to 80 of 80 - powered by ASPPlayground.NET Forum Trial Version
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Re:I keep killing my rotifers
Saturday, March 14, 2015 10:04 PM
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Don't let fear and common sense stop you! =]
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Re:I keep killing my rotifers
Saturday, March 14, 2015 10:13 PM
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Mindy, it sounds like I'm diluting the same. 1l of culture to 1l nsw.
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Re:I keep killing my rotifers
Sunday, March 15, 2015 11:43 PM
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i can't really see the difference in the pics. The videos are easier to see. I'm thinking that if you wrote something on the bottom of the bucket, and can read clearly, then you know you need to feed. If you can't read it clearly, then they have enough food. That is assuming there is no gunk on the bottom.
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Re:I keep killing my rotifers
Monday, March 16, 2015 2:26 PM
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How are you treating your NSW? Are you collecting it yourself?
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 10:21 AM
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Good point Gresham, I forget many Americans have access to NSW. People seem to get new SW and NSW mixed up though...
Originally Posted by president89
i can't really see the difference in the pics. The videos are easier to see. I'm thinking that if you wrote something on the bottom of the bucket, and can read clearly, then you know you need to feed. If you can't read it clearly, then they have enough food. That is assuming there is no gunk on the bottom. I mean this in the nicest way possible, are you at all color blind? The top photo is brown, and the bottom photo is green. Colorblindness would be a challenge in this case! You're right though, when there is food left in the culture the culture appears blurry.
Don't let fear and common sense stop you! =]
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 11:42 AM
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I am color blind. And I meant new artificial saltwater...sorry for incorrectly using the wrong acronym.
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 12:23 PM
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Dang... I thought I was on to something. FWIW judging by color is hard at any rate. The more rotifers, the browner the culture, the greater need for feed. I started on a color wheel for judging feed and densities and its really freaking tough. My monitor calibrator is old now and doesn't work with the new Windows
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 3:56 PM
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I think it sounds like you are going to have to use a fixed quantity fixed time method. Might not be as efficient but it will help you keep a steady culture of rotifers. The 11ml had been discussed extensively above but, in my opinion most people don't need max production. Being that you just got starter cultures I would add one to 4G of water in your CCS and add 2ml of RGC twice a day. Once a week remove approximately 1G of culture water (and rots) and replace it with fresh artificial saltwater (aged 24 hours minimum). Also rinse your rotifer floss weekly. Once a month SIEVE out 3G of the water and replace with fresh artificial seawater (aged 24hrs min). Keep your specific gravity at .005 less than your system. Rotifers can take a .007 shift fine. I think it you just go with the system above exactly you will have a steady supply of rotifers. If you use RG+ then feed 1ml twice daily. As mentioned earlier I think due to bad luck earlier you are worrying too much and over complicating things.
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 4:51 PM
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The above recommendation... at that point you pretty much wasted your money on the CCS... just use a rigid airline and bucket. The floss won't help much if only cleaned once a week, with a water change only once a month. Just to clarify.... Add 1 liter rotifer culture to 13l of culture water to arrive at 14l.
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 4:55 PM
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I will try above. I am not going to use the ccs for now. The 24 hour aged SW might have been an issue. I don't always wait that long....
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 5:10 PM
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me neither, but its never caused me an issue. I do use air plus powerheads when using ASW that I need to use quickly.
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 6:45 PM
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Yes I agree it's not needed to age 24hrs, simply intending to imply properly mixed. I guess I could have just stated it that way. Just trying to be as specific as I could and create a conservative protocol to raise a decent amount of rotifers but by no means be as efficient or as productive as the proven and professional protocols Gresham recommended. I very seldom needed rotifers consistently or in large batches (1 million/day) so I would roughly follow the above to "ramp up" and then let them "coast down". As for the floss, should it be cleaned daily Gresham? Sorry for the bad info if so. I will fully admit I pulled the weekly idea from ... Well... places. (I understand some may have the opinion that this is where all my info comes from!)
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Re:I keep killing my rotifers
Tuesday, March 17, 2015 7:11 PM
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Can you post some pics of your setup and water like Eastereggs did? Maybe someone will see the ah-ha thing we are struggling to find.
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Re:I keep killing my rotifers
Wednesday, March 18, 2015 10:25 AM
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Originally Posted by president89
I am color blind. That complicates matters! I do think using small cultures will help you see the difference. I think your best bet for judging clear vs not clear is going by clear vs blurry looking. Maybe if you use small white buckets and put a few 1/2" black dots on the bottom with a Jiffy marker so you can see if the dots are clearly seen or blurry will be the best bet for you. Honestly, I think your only problem is that you have been overfeeding. Rotifers are easy and this thread is really making them out to be complicated. Here's an old thread I started Small Scale Rotifer Production for the Lazy Man (or Woman). The method works quite well. Don't feed the 2.25 mL per L (of RotiGrow Plus) I refer to though, that was explaining how dense the cultures can be using this method. If you follow this method and feed 0.5 - 1 mL per liter of culture you can't go wrong.
<message edited by EasterEggs on Wednesday, March 18, 2015 10:53 AM>
Don't let fear and common sense stop you! =]
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Re:I keep killing my rotifers
Wednesday, March 18, 2015 10:42 AM
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I've been underfeeding this time on purpose.. and so far so good. I'm adding 1ML RG+(equal parts diluted chloramx solution) or 2ML RGC at the very most. So far, so good. I am going to get some small white buckets, and put a letter at the bottom with a sharpie marker. I think blurry/clear is the way to go when you are color blind.
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Re:I keep killing my rotifers
Wednesday, March 18, 2015 10:54 AM
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Underfeeding sounds like a good course for you to be on.
Don't let fear and common sense stop you! =]
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Re:I keep killing my rotifers
Monday, March 23, 2015 8:27 AM
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Originally Posted by waldend
Can you post some pics of your setup and water like Eastereggs did? Maybe someone will see the ah-ha thing we are struggling to find. Here is a video. I haven't moved to smaller buckets yet, and water volume is higher than I want. https://www.youtube.com/watch?v=bfRxV76q0Hk
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Re:I keep killing my rotifers
Monday, March 23, 2015 10:07 AM
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Originally Posted by president89
A couple things that are very different from what I do (and I say it that way because it may not be "wrong" exactly) is you're using a lot less air than I do, and I've found my rotifers have done a lot better with clear lids (I use a piece of acrylic). You're using about the same amount of air that I use in my little cultures. Of course volume is a big difference too. EDIT: Looking at my video and then looking at yours, I can see clearly that I am actually using MORE air in my bucket than you are using in yours and mine is about 1L in the video and yours appear to be about 4 gallons each. That's significant.
Don't let fear and common sense stop you! =]
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Re:I keep killing my rotifers
Monday, March 23, 2015 12:23 PM
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I agree, that's not enough flow. FWIW you are using air to move water, not put oxygen in that water (that is an artifact of it though).
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Re:I keep killing my rotifers
Monday, March 23, 2015 3:47 PM
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