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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:01 PM
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 Originally Posted by Caesra
Interesting setup, how well does the teddy bear grow with light that level?
LOL the monkey did well until it met it's untimely demise when the puppy arrived.... such is nature! A note on the setup, I have *extremely* limited space so I needed to come up with something that fit and was functional, but was also efficient, flexible, and easy to access and maintain. I've been extremely happy with this design as I can very quickly add, subtract, or move shelves as my culture needs change; I can also change the bulbs to allow for differing spectrums from one side to the other, currently the right most is running 6500K's the center is at 6500 and 3600 and the left most 3600, this way I can somewhat play with light spectrum. I did at one point cut some separators to slide between fixtures so that I can isolate the lighting for cultures as I was playing with spectrum... I've since waylayed that due to time and *meh* factor. Right now the bottom shelf resides about a quarter inch from the bottom of the bulbs allowing me to keep zooplankters (currently co-cultures of pseudo, T. japonicus, and rotifers) with some ambient light but not a full on photonics barrage. And of course since all the trips came up... I've got an empty shelf now.... but that wont last for long
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:07 PM
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That is my delema, I have already confiscated alot our house for fish stuff (about 1500 sq ft so far) and the wife gave me permission to suck up another 1500 sq ft or so, but I feel completely guilty so I am trying to work within the space I am already using...maybe the reef tanks will have to go =P. So I am trying to figure out if I will be good just to plop these on my shelves I bought for my rotifers, which is basically a 5 level plastic shelf unit. I am feeling more comfy with the rotifers now, so I don't need to keep 6 cultures going like I am now. No direct lighting on it..actually no lighting atm accept for when someone is in the room. I am basically completly out of space. I have a small area above my fridge in my breeding area, but I was going to use that for fry containers for clowns. There is some family storage in the 'fish room' I could probably confiscate, but again..trying to avoid doing that. So these are my many questions as I try to figure out how to put more stuff in a small area. It sounds like I will be fine doing them on the rotifer shelves and adding a T2 light I have sitting around for these.
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:15 PM
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If it gives you some idea, I'm currently running 23 gals. of O. marina and Apocyclops cultures: 10 gals. (five 2 gal. buckets) of O. marina and 13 gals. (one 5 gal. and four 2 gal. buckets) of Apocyclops. I do 2 gallons of copepod harvest per day and 2 gallons of dinos to replace that water. Then 2 gals. of new saltwater to the dino cultures. The 2 gal. copepod cultures are on a wheeled printer cart that I can move around. The 5 gal. is in a free spot in the fish room. The dinos are on a shelf in another room.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:21 PM
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:34 PM
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Originally Posted by slosht
Originally Posted by Rook
Hmm, so I need to figure out how to keep the heat up a bit. My basement is 65-68.
Jeremy, I'm not sure if this would work, but what about putting one of those reptile heating pads under the containers? They have these flat hard plastic pads specifically for reptiles. It might be a way to bump the heat up just enough. I'd put some foam down under the pad as insulation. You know, I was thinking that its too bad there is not an underground heating devise like under floor heating, and you found it. Interesting idea, I might be too concerned with possible problems spilling the water, which is sure to happen. I was considering finding a large, shallow storage container, like those cloth containers that fit under your bed; fill with water, place buckets in water, place heater in water. Not idea, needs to be watched to ensure it does not evap.
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:34 PM
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yeah I never got along with buckets... I like my sterilite tupperware containers and smaller plastic jugs. I'm a fan of multiple smaller cultures, with the exception of my Nanno which sees 3 gallon carboys most of my stuff is in 2 quart tupperware or gallon water jugs, if I feel like getting intense I shift shelves and kick up mason jars, I can fit about 25-30 on a shelf and kick in a ton of shelves due to the low height In edit, seeing the post regarding temp... the $8 concrete tubs (black plastic tubs) from home depot make excellent water baths, this is what I was using when I was attempting to study the Nassarius snail. Evap does get you but if your culturing you need to spend a few minutes per day anyhow, so it's just a matter of remembering to top it off.
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:50 PM
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Originally Posted by Andy is the bucket man. I like that. 
Originally Posted by reptile heaters I thought of that, too, but I don't trust that they'd play well with saltwater.
Originally Posted by fill with water, place buckets in water, place heater in water. Water baths are a good idea (the big boys tend to use water bath tables to keep temps stable). They are heavy, though, and you have to find a good container the right size. You can just use fresh water for them.
Originally Posted by kick up mason jars I used to be all about the glass, but they are really expensive compared to the amount of water they hold. Plus, you know, I'm kind of a klutz. I don't know if you've noticed, but klutzes and glass don't mix well. And they are always built so that they are a pain to clean for some reason.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture these pods
Tuesday, July 26, 2011 9:58 PM
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^^^ sooo many comments, soo many ways to kill a thread... I've definately broken my share of mason jars, and it's not uncommon for the floor of my small space to get errrm... planktoned... I suppose one could do it with plastic and such given the great myriad of offerings available now (I love us plastics by the way) but the mason jars are there, handy... waiting... When I flew out for the workshop I took a couple of 16 ounce low cut PET containers I picked up from US in the event that I would do some lake collecting (yes I get into FW copepods too) and they've always served me well. The core reality is that culturing takes a *ton* of work and attention, modifications are born of necessity and trying new things that you think of on your own to tackle your own specific issues is the best way to go still. I love threads like this as they point out things I never thought of.... it's possible to do a heat rock (they are waterproof fyi) in the back of a shelf and perhaps screen the front with some plastic to hold the heat in?? With the AC kicked up I am always fighting temp here, lower temps cause problems as do higher... ideas in this arena are always well appreciated!! One of the best things I have done with my new (well new-ish) setup was to push the culture racks in the spaces behind the tanks and back wall where the heat collects... in front of the tanks may be 68, behind due to the false wall and such is a pretty stable 72
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Re:Supplies you need to culture these pods
Wednesday, July 27, 2011 7:49 PM
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Re:Supplies you need to culture these pods
Wednesday, July 27, 2011 8:29 PM
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ok, so how much volume of each do we need? As I understand it, we can raise the o marina off of paste. These feed the pods. Will 1g of dino culture feed 1g of culture pods? just trying to undestand the ratio of volume need for each culture.
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Re:Supplies you need to culture these pods
Wednesday, July 27, 2011 10:15 PM
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So many good ideas in this thread. Looking around for suitable culture vessels, I spotted my cereal container on the counter. It's about 2 quarts, plastic, and has a lid that seals most of the top but I could leave the pour spout open to get an airline in. They are narrow, and could stack quite a few across a shelf. Has anyone tried using them before? I assume I'd have to increase the air flow through them to make up for the lack of surface area, but does anyone think that is going to be too much of a detriment?
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Re:Supplies you need to culture these pods
Wednesday, July 27, 2011 10:16 PM
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actually intersting idea. they just cost more than say an average 5 or 2g bucket
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Re:Supplies you need to culture these pods
Wednesday, July 27, 2011 10:39 PM
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few ideas here... can translate to multiple species fairly simple... and it's sorta what I use... The arrowhead 3 liter water jars have a dimple in the bottom that helps with flow, can be used three times before fouling and either cleaned and re-used or discarded... 1$ each. For zooplankters I prefer my sterilite snapware containers http://www.usphome.com/catalog/item.aspx?itemid=74733&catid=963 the vent makes it a handy hole for the airline, which keeps the open tops a little more sealed... helps a lot.
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 12:57 AM
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And yet, those buckets sure are cheap.... I may need to try a closed-top system when I try Parvocalanus again but the Apocyclops truly are vigorous enough not to really need them.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 8:26 AM
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I have iso and nanno and some rotifers coming now.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 9:45 AM
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Very cool. Good luck with them. I hope you have better luck with Iso than I've ever had.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 10:03 AM
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FWIW, I keep several backup cultures of Apocyclops in simple, 500ml and 1000ml Erlenmeyer flasks with no aeration. Of course, I am feeding them live ISO, which probably helps with the DO.
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 11:23 AM
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 1:29 PM
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I have a "standard" arrangement of containers I use for different things. For my Berghia And small/starter cultures I use the 1G square with like 6" round lid Tupperware containers. They are easy to handle and clean. For Rotifers I use a 5G bucket and I also have some 10G buckets to use. Same footprint as 5G but taller. For all of them I drill a hole right in the center just big enough for a rigid airline to freely drop in. This allows me to secure the lids since I have a young child and prevents excessive evaporation. My thinking for this project is a 10G For the O. Marina and 5G for the Apocyclops.
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Re:Supplies you need to culture these pods
Thursday, July 28, 2011 8:21 PM
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hrmm... reading through the spacial concerns in this thread has hit me with an idea for a new culture rack... similar to the slide out DVD racking I built in the bedroom closet for my wife. Think slide rails at top and bottom of a shelving unit that allow them to slide *out* of a cabinet.... More to come, I still have at least one set of the slide rails in the man-shed and a second set is a HD trip away.. maybe I'll do a proof of concept.
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