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•  Culture Journal, Species: Tigriopus californicus

  Change Page: 12 > | Showing page 1 of 2, messages 1 to 20 of 30 - powered by ASPPlayground.NET Forum Trial Version Reports tied to this Journal Author Message EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Culture Journal, Species: Tigriopus californicus Saturday, April 14, 2012 9:05 AM (permalink) Culturing Journal DataSheet General Species:  Tigriopus californicus; "Tigger Pods" Species description:  250-1700 microns red copepods that swim in jerking motion.  Culture source (link if possible):  Reed Mariculture www.reedmariculture.com Culture Establishment Date:  April 10, 2012.  Re-established November 3, 2013 (photo at April 2, 2014). Continuation Date:  October 9, 2014. Culturing Vessel Details Salinity:  Varies between 1.025 and 1.030. Temperature:    Ambient fish room temperature 76-80F. pH:  Not tested Vessel description:  Mostly 15x15x10" clear plastic Sterilite storage containers.  Start with only an inch of saltwater in them.  Volume will increase as density increases. Lighting description:  Ambient room light from overhead fluorescent. Lighting cycle:  14 hours light, 10 hours dark. Aeration description:  None Methodologies Split methodology:  None. Culture medium description:  ASW, not sterilized.  RotiGrow Plus fed when culture becomes colorless, and ClorAm-X solution used at same volume - about 1 mL per gallon of culture. Cell count:  Started with 2000-4000 critters in the 6 oz bottle. Reference links:   http://reefnutrition.com/tigger_pods.html Additional Information Notes:  Biiiig difference when I switched from a clear tupperware container to the painted aquarium. <message edited by EasterEggs on Wednesday, February 4, 2015 9:29 AM>   #1 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Saturday, April 14, 2012 9:33 AM (permalink)     #2 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Saturday, April 14, 2012 7:52 PM (permalink) Please post suggestions if you see me doing something wrong.    #3 GreshamH Total Posts : 757 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Monday, April 16, 2012 7:24 PM (permalink) 2 - 4K per bottle   #4 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Tuesday, April 17, 2012 9:16 AM (permalink) Thanks Gresham, I updated the numbers.   Well, I could be mistaken, but I think the numbers are increasing.  I'm up to about 750 mL of saltwater in the culture - that bowl is almost full now.  I'm going to siphon the bottom today because I put too much RGcomplete in there a few days ago, and I don't think dead phyto should be in there that long.  I'm going to split the culture into two today too.   How prolific should these copepods become?  Should I be doing harvests to keep the population young like we do with rotifers?  I haven't removed any of the population yet.   #5 waldend Total Posts : 604 Reward points : 1855 Joined: 10/5/2010 Location: Lafayette, IN, US Status: offline MBI: 310 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Tuesday, April 17, 2012 10:34 AM (permalink) I didn't maintain my culture of these very scientifically. I simply put some in a 1G jar with lid ( small hole to allow some air) on a window sill. I might occasionally add a few drops of Roti-Grow plus and maybe a couple flakes of formula one here or there. I did the same with another batch not in the window sill and the population was much higher in the jar exposed to sunlight. The natural phyto I believe was the key. When the population was small the jar turned slightly green ( I never added live phyo, hence the "wild" description). Sorry I don't have exact densities but the difference between the two was night and day. I had planned to do an outdoor culture like Tals but never did prior to closing down. One thing I forgot - I did add S rotifers to the same jar. I would have to research but I did it after reading a thread from KathyL where she stated she co-cultured.   #6 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Tigriopus californicus Tuesday, April 17, 2012 11:44 AM (permalink) yes, if you don't mind having rotifers in there, they do quite well together.  If I recall correctly, I maintained a one bucket culture with a sponge filter and daily additions of RG for months with seldom a removal or water change.   #7 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Tuesday, April 17, 2012 7:25 PM (permalink) Yeah, these guys really seem bulletproof, eh?  I don't want to put rotifers in with them, I would like to maintain separate cultures.    Thanks for the tips on the natural sunlight Dan, I will try that out.  Is it direct light shining right on the container or just indirect, close to a window?   #8 Fishtal Total Posts : 5467 Reward points : 2906 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Tigriopus californicus Tuesday, April 17, 2012 7:39 PM (permalink) I do mine outside with great results. See my journal. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #9 waldend Total Posts : 604 Reward points : 1855 Joined: 10/5/2010 Location: Lafayette, IN, US Status: offline MBI: 310 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Tuesday, April 17, 2012 8:21 PM (permalink) I had mine sitting right on the window sill almost touching the glass. It was winter though so temperature wasn't an issue. Depending on the room temp I might try to place it further away from the window to avoid the heat but close enough to get direct light.   #10 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Wednesday, April 18, 2012 8:35 AM (permalink) Ok, thanks Tal and Dan!   #11 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Friday, May 11, 2012 8:54 AM (permalink) While I was gone to Kauai the Copepods almost died out.  My instructions weren't clear enough about adding water volume (to keep water quality up).  I was left just with a small population of small Tiggers.  Now I've been home for a week the little oens have definitely grown (I hear the reproduction cycle is 3 weeks?), but they are no longer red.  I've heard this happens when you culture them yourself.  How do I get them red again?  TDO maybe?   #12 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Tigriopus californicus Friday, May 11, 2012 12:40 PM (permalink) just keep feeding them.   #13 GreshamH Total Posts : 757 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Friday, May 11, 2012 2:33 PM (permalink) In the wild, IME populations tend to increase with higher salinity... IMO its a fight for the continuance of the species.   Quality phytoplankton is what makes them red in the wild, and here at Reed.    #14 Fishtal Total Posts : 5467 Reward points : 2906 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Tigriopus californicus Friday, May 11, 2012 4:56 PM (permalink) Mine are red: http://www.mbisite.org/Forums/tm.aspx?&m=46275&mpage=1 http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #15 waldend Total Posts : 604 Reward points : 1855 Joined: 10/5/2010 Location: Lafayette, IN, US Status: offline MBI: 310 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Friday, May 11, 2012 7:46 PM (permalink) Mine would come and go with the red.   #16 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Sunday, May 13, 2012 9:04 AM (permalink) Hmm, ok I will just carry like I am then.  I'm using 1.025 salinity, although I should test and see what it is evaporating to.  Probably higher, I know they like higher salinity, so I haven't been too worried about evaporation.  I just moved them into a 2 gallon shallow clear Rubbermaid container yesterday, less than an inch of water so far though.   #17 GreshamH Total Posts : 757 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Sunday, May 13, 2012 2:21 PM (permalink) Originally Posted by EasterEggs Hmm, ok I will just carry like I am then.  I'm using 1.025 salinity, although I should test and see what it is evaporating to.  Probably higher, I know they like higher salinity, so I haven't been too worried about evaporation.  I just moved them into a 2 gallon shallow clear Rubbermaid container yesterday, less than an inch of water so far though. 1.025 is specific gravity, not salinity fwiw.  Salinity is measured in PPT.. IE. 35 ppt (1.026 Specific Gravity). We keep out Tigrioupus at 35 ppt and ship at 35 ppt as well.   We've found clear is not a good solution.   #18 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Sunday, May 27, 2012 7:55 AM (permalink) Yeah, I was being lazy about the salinity vs specific gravity...too many letters.  I suppose I should have said SG.    I forgot you said to not use a clear container, and I haven't transferred them to a better option.  I need to find something else.   They population isn't increasing with much vigor.  Anyone know what kind of density I should expect?  Maybe they just want more space.  They are about half as dense as the very first photo I posted of them.   #19 EasterEggs Total Posts : 1946 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Tigriopus californicus Sunday, June 17, 2012 8:30 PM (permalink) I looked in the container today and I notice that the copepods are a bit denser than they have been.  I figured I should clean the surface (I just lay a dry piece of paper towel on the surface and the gunk sticks to it).  I also tested the specific gravity...1.070!!  It was almost off the chart in my refractometer!  So apparently they are very salt tolerant!  I added some water to lower SG a bit, and will bring it back to somewhat normal numbers.  Haha!  After reading Hoff's book (again) I think I will try moving half the culture into a 5 gallon tank with black sides and a white bottom. Don't let fear and common sense stop you! =]   #20 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: 12 > | Showing page 1 of 2, messages 1 to 20 of 30 - powered by ASPPlayground.NET Forum Trial Version

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