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Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, April 25, 2012 7:25 PM
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Breeding Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Amphiprion chrysopterus, Yellowfin anemonefish, Bluestripe anemonefish Social Structure: pair Size of Individuals: Large, about 4 inches for the female Age of Individuals: Unknown, wild caught , origin Fiji Date added to Tank: April, 2007, these are not my fish. Kevin Steinmann owns them. He lives about 2 hours from me by car. Broodstock Tank Details Size of Tank: 125 gallon tank on a 300 gallon system Substrate Details: Reef tank with live rock, sand, corals, etc. Filtration Details: S.G.=1.026, live rock, protein skimmer Water Changes: Water Temperature: 78-82F Lighting: 175W metal halide Lighting Cycle: 12hours day, 12 hours night Other Tank Inhabitants: 2 tangs and 2 blue assesor, corals, invertebrates Broodstock Feeding Details Food Types: Feeding Schedule: Spawning Details Date of First Spawn: Feb, 2011 Spawn Time of Day: unknown Dates of Consecutive Spawns: KS on June 1, "They have spawned a total of 7 times. They will lay 2 or 3 batches within a couple of weeks and then that's it. I think that temp plays a key role. Most notably a drop of 2-3 degrees of their normal temp. for several days seems to get them in the mood." This spawn was on approximately 4/19/2012, but we are not sure. Courtship Details: female becomes gravid, but otherwise unknown details Egg Size: 2mm by 1mm Egg Color: orange, then brown, and then silver, just like all clownfish eggs Egg Count: around 200 Hatch Details Hatch Date: 4/27/2012 Hatch Time of Day: suspect around 5:30-6 am, not sure. # Days after Spawn: 8 or 9 not sure as we are not sure of the spawn date. Larvae Description normal clownfish looking larvae, about 4mm long, transparent with eyes, tummy, tail. They seem a bit more energetic than ocellaris. Consecutive Hatch Dates: some premature hatchlings on 4/25 and 4/26, but the main hatch happened on 4/27/2012 . (This pair does not spawn on a timetable.) Larval Tank Details Temperature: 80F Size of Larval Tank: 5 gallons in a 15 gallon BRT Substrate Details: bare Other Tank Decor: heater, airstone, seasoned sponge filter at around day 7 Filtration Details: none at first, small doses of chloramX with phytoplankton added. Seasoned sponge filter added after 1 week, bottom siphoning done a couple of times. Lighting: Marineland LED strip, blue. on rim of tank, otherwise ambient lighting. After a few days, switched to 18 watt fluorescent bulb with reflector mounted just 10 inches above the water, and only for day. I used the blue LED strip at night for the first 12 days or so. Then I used the 18 watt bulb for 14 hours daylight, and darkness for 10 hours night time. Lighting Cycle: 14 day, 10 night Water Changes: addition of 1 gallon per day of fresh saltwater for the first week. Rotifers were removed with a gallon of water 3 times during the first week. Rotifers were added after the addition of the sponge filter about 3 times. Top up with fresh unsalted water as needed to compensate for evaporation. Larval Feeding Details Food Types: initially rotifers raised on RG complete, enriched with Instant Algae Isochrysis and N-Rich . Apocyclops panamensis, Otoheim A, and Newly Hatched Brine Shrimp. Feeding Schedule: 30-50 rots /ml, as needed . At day 2, added some Apocyclops panamensis. Added more 2 days later. At day 13, added newly hatched brine shrimp, but not much, and every day thereafter. Tried Otoheim A size pellet feed each day starting on Day 9 or 10 but no real evidence of it being consumed. Just a tiny sprinkling. Metamorphosis/Settlement Date of Settlement Start: 5/12/2012 Days after Hatch: 15 Date of Settlement End: 5/16/2012 Description of Fry: Dark body, clear nose, Black spots on body, clear long fins, faint white headband, rather thick. Body 1 cm long approximately. Mostly hangs out at the meniscus.
Grow-Out Tank Details Temperature: 81F Size of Grow-Out Tank: 20 gallon Substrate Details: none Other Tank Decor: heater and sponge filter for a while, and then system was turned on Filtration Details: sponge at first then K1 kaldnes media, after filter bag, then heaters and UV 40 watt on 240 gallon system Lighting: string of LED, 2 watts per foot Lighting Cycle: 14 hours day, 10 hours dark Water Changes: about 10% weekly Size at Transfer: about a quarter to an eighth of an inch Age at Transfer: about 4 weeks Grow-Out Feeding Details Food Types: Otoheim pellet food Feeding Schedule: 4 times per day Additional Information "Ron Popeil's" attempts and success from 2007: http://www.reefcentral.co...owthread.php?t=1032143 (No Pictures or Videos in the Section Please) Miscellaneous Information: note: 1st spawn was 4 mnths after moving from a 72gal fowlr to the 125 reef with much more space and much higher water quality. They have spawned a total of 7 times. They will lay 2 or 3 batches within a couple of weeks and then thats it. I think that temp plays a key role. Most notably a drop of 2-3 degrees of there normal temp. for several days seems to get them in the mood. If they spawn with this cold snap i may try to hook up a chiller and see if i can force it. You will be required to provide photographic or video evidence in this thread of each event submitted for the MBI Program.
If your thread does not contain these photos the MBI Committee will not be able to approve your reports. PHOTOS AND VIDEO S MUST BE PLACED IN ADDITIONAL POSTS, NEVER IN THE FIRST POST IN A JOURNAL.
<message edited by KathyL on Saturday, July 21, 2012 12:29 PM>
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, April 25, 2012 7:31 PM
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My friend, the saltwater savant, has a pair of Bluestripes spawning in his tank. He is sharing the eggs with me. I got a bunch of them today, and so far, 3 eggs have hatched. I think the rest will hatch tonight. I'm excited. I can't claim a spawning report, but a hatching report will do.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, April 25, 2012 8:07 PM
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Congrats! Can't wait to see little ones.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, April 25, 2012 8:22 PM
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Thanks Dan, me neither.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, April 25, 2012 9:28 PM
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WOW - REAR THESE! LOTS!
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, April 25, 2012 9:41 PM
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Um, OK.  The hard part is getting them to spawn, and Kevin is the Man.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Thursday, April 26, 2012 10:24 AM
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Kathy, do you know what geographic location they're from? I ask, because there's a fair amount of geographic variation in this species (eg: white tails vs. yellow tails). (Matt's hoping they're a yellow tail form that has extended caudal fin lobes...kinda like a lyretail). Oh, and when do we get to see pictures of the broodstock? 
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Thursday, April 26, 2012 1:58 PM
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I'll see what I can do for pictures. i think these guys have yellow tails, but I am not certain. Kevin has a similar pair of fish of a different species…., and I recall that one of the pairs has a lyre tail, but I could be wrong. Kevin has done aquarium maintenance for a zillion years, and he quaranteens his customers' new fish for them at his home. He is amazing at getting fish to live that would not otherwise do well. So he orders in fish from his connections, and can at times order in something for himself. That's all I know about where these came from. Edit: The broodstock are from Fiji.
<message edited by KathyL on Saturday, June 2, 2012 5:52 PM>
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Thursday, April 26, 2012 2:08 PM
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I'll see what I can do for pictures. Three hatched last night, but I could only find one this morning. Their little hearts are still beating, so tonight is the night!
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Friday, April 27, 2012 6:49 AM
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only15 last night…. I checked this morning before my morning walk, and I maybe found one….. Thought I would give up on this hatch. I looked for more swimmers after the walk, and I didn't find any. They could still be in there. The BRT is very dark inside, and tranparent larvae are hard to see. I went to clean up the hatcher and I thought I would check for late hatching larvae. I turned off the air, and lo and behold, they all hatched. Except for about 10 eggs, they all hatched. I am thrilled! They are very energetic despite having spent the night in the turbulent hatcher. Now hours later in the BRT, they seem to be eating rotifers and doing fine. I have a blue LED light strip over the tub, the lights we got as a door prize at MACNA (I really like them as they are waterproof). Also, I have an airstone and a heater. Rotifers at about 30-50/ml, 5 gallons of water, 30ppt. Ill be making that a bit more dilute. I am feeding the tank with Isochrysis from Reed as green water as well as RG complete. After some conversation with Kevin, we are both thinking that these guys hatch later in the evening or early morning. I have a vague recollection that I checked the hatcher this morning, before the walk, and looked at an egg under the scope, but I may not remember it well, as I was barely awake at the time. If so,though, it means they hatched sometime between 5:30 am and 7:45. Keven, who was up all night, thinks that they hatched around 3:30 am, and that previous hatches from this pair did not occur before midnight. Also, to answer Matt, the parental tails are yellow, the region they came from is Fiji, and their first spawn was around April of 2007. They are not lyre tailed. Darn. But I'll take what I can get. YAY!
<message edited by KathyL on Friday, April 27, 2012 12:14 PM>
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Friday, April 27, 2012 1:26 PM
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Here's a video of one of the eggs, yesterday: 
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Friday, April 27, 2012 1:28 PM
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And one of the larvae, today: 
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Friday, April 27, 2012 9:18 PM
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They are all swimming , and seem to be eating. Hard to see them in a BRT. I'm leaving the light on overnight.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Friday, April 27, 2012 9:19 PM
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Awesome!
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Saturday, April 28, 2012 11:56 AM
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The larvae are still swimming energetically, but don't have fat tummies despite an abundance of rotifers. I guess we'll know in a couple of days if they are eating or not. I'm feeding the rotifers in the BRT mostly Isochrysis, because a few drops of the new bottle of RGcomplete seems to create an oil slick.
They seem to really like the blue light, as they are concentrated underneath it.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Saturday, April 28, 2012 4:24 PM
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After looking at some of the babies under the microscope, I'm afraid that they aren't eating. I decided to add some Apocyclops panamensis to their tub. It's a cyclopoid copepod that is slightly larger than a rotifer, but has copepodites that are smaller. And they are very nutritious. Here's a picture of a Day 1 larvae:  Looking at the shiny belly region, perhaps they are eating....time will tell for sure.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Sunday, April 29, 2012 8:31 PM
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Day 2.5
Lost a lot, so I guess they are not eating. Still have about a dozen. Some are eating. Kevins half of the nest is down to 3. I wonder what metamorphosis will be like.
I measured one survivor today, and it is about 4mm, the same length as one of my ocellaris that is about ready to metamorphosize today. Maybe they don't grow longer in the larval stage, just grow in width. These larvae seem much smaller all around than ocellaris larvae at this age. I wonder if this species needs S-rotifers or copepod nauplii in order to survive.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, May 2, 2012 10:02 AM
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And then there were six.
And then there were three.
And then there were two today.
And now we wait.
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, May 2, 2012 10:37 AM
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Bummer to see you losing them. I have faith you will figure it out!
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Re:Breeding Journal, Species: Amphiprion chrysopterus
Wednesday, May 2, 2012 10:03 PM
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Thanks, I think the large die off is either broodstock nutrition, or that rotifers are too large for the babies. They are really different from ocellaris.
Kevin also has 2 larvae, and no meta yet. So 4 survivors so far.
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