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Breeding Journal, Species: Priolepis hipoliti (Rusty Goby)
Thursday, January 16, 2014 8:47 AM
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Breeding Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Priolepis hipoliti (Rusty Goby) Social Structure: Pair Size of Individuals: ~1 inch Age of Individuals: Unknown Date added to Tank: 10-29-2013 Broodstock Tank Details Size of Tank: Mesh breeder basket in a 40g "breeder" on a 200+ gallon system Substrate Details: None in the basket Filtration Details: Live sand, live rock, protein skimmer, bioballs Water Changes: Infrequently Water Temperature: ~80F Lighting: Fluorescent strip light Lighting Cycle: 6AM to 9PM Other Tank Inhabitants: None in the breeder basket Broodstock Feeding Details Food Types: Live blackworms, Hikari mysis, frozen mash Feeding Schedule: Two or more times a day Spawning Details Date of First Spawn: 1-16-2014 Spawn Time of Day: Unknown, sometime between 7AM and 5PM Dates of Consecutive Spawns: 1-21-2014 Courtship Details: Not witnessed Egg Size: Very small, ~0.5mm, maybe less Egg Color: clearish yellow at first Egg Count: Not counted, but at least 300 Hatch Details Hatch Date: 1-15-2014 from spawn of unknown date (I wasn't checking for eggs before I caught the larvae) Hatch Time of Day: Very shortly after lights out # Days after Spawn: 5th night after spawn Larvae Description: Very small at ~2mm, pigmented, likely attracted to light since they were caught in a larval snagger otherwise by dumb luck hundreds ended up in the snagger (also very possible) Consecutive Hatch Dates: 1-20-2014 Larval Tank Details Temperature: 80F Size of Larval Tank: 10 gallon Substrate Details: None Other Tank Decor: Heater and airstone Filtration Details: Live phyto Lighting: Fluorescent strip light Lighting Cycle: 6AM to 9PM Water Changes: Larval Feeding Details Food Types: Gymnodinium, Heterocapsa triquetra dinoflagellates Feeding Schedule: constant Metamorphosis/Settlement Date of Settlement Start: Days after Hatch: Date of Settlement End: Description of Fry:
Grow-Out Tank Details Temperature: Size of Grow-Out Tank: Substrate Details: Other Tank Decor: Filtration Details: Lighting: Lighting Cycle: Water Changes: Size at Transfer: Age at Transfer: Grow-Out Feeding Details Food Types: Feeding Schedule: Additional Information (No Pictures or Videos in the Section Please) Miscellaneous Information: You will be required to provide photographic or video evidence in this thread of each event submitted for the MBI Program.
If your thread does not contain these photos the MBI Committee will not be able to approve your reports. PHOTOS AND VIDEO S MUST BE PLACED IN ADDITIONAL POSTS, NEVER IN THE FIRST POST IN A JOURNAL.
<message edited by shannpeach on Wednesday, April 16, 2014 2:40 PM>
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Thursday, January 16, 2014 8:52 AM
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Pic of the adults:  Pretty sure the female is the one closer to the PVC pipe since that one is a bit more plump around the middle This morning I checked the snagger expecting to see some snapping shrimp larvae and found these instead!     About a week ago (maybe two) they were moved from the QT tank into the 40 breeder. I had checked for eggs (at least I thought I did) and have peeked with the flashlight a couple times but never saw anything. Must not have been looking hard enough! I will have to check more thoroughly in the future. There were at least 100 larvae! They are so so tiny though, ~2mm. I put them in a 10 gallon tank and with some rotifers and added a bunch of 53uM and larger Euterpina copepods. I added some tetraselmis (not the best alga nutrionally, but it was all I had at the moment) to tint the water green.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Thursday, January 16, 2014 6:52 PM
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They must have spawned today and holy moly. Here is a pic of the eggs in the PVC tube:  And they must have run out of space because there were also a whole bunch of eggs on the clam shell:  Hundreds and hundreds of eggs I would say, and they are TINY. The larvae are still swimming around and they also are just so TINY.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Friday, January 17, 2014 10:18 AM
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I pulled a couple to take some pics last night to hopefully see if they are eating  This one not eating? I can't really see any food in there...   Eating? Or just a little bit of yolk or oil globule thing? I have no idea. All I know is that this morning there were still some alive. I left the light on over them last night and will probably continue to do so as long as they are alive...Which may only be through today! I can't get over how small they are
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, January 20, 2014 9:19 AM
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Tuesday, January 21, 2014 8:51 AM
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Hatch last night! And it was very shortly after lights out (within 30 minutes probably!) I think I sort of messed things up by going down there with a flashlight and trying to get some out right away. They seem mildly attracted to the light (or perhaps just was random?) but they are incredibly evasive and can swim surprisingly well, so I hardly got any with the baster and just gave up. This morning there were very few in the snagger...Next time I am going to try an artificial hatch perhaps
Anyway, I did get a few larvae. I don't have anything to really offer them in terms of food, but I decided to do a starvation trial and a "let's see if this works" trial. I have two small cereal containers (ummmm....will have to check the actual volume. I would estimate 1.5-2 liters). One container is just some larvae in broodstock water and I added nothing. The second is larvae in broodstock water with tetraselmis (the largest phyto I have currently) and this morning I sprinkled in some of the smallest golden pearls I had (50-100uM). I think those will still be too big, but hey, why not try? I am not aerating the containers and they are placed on a germination mat controlled by a finnex heater controller thing (the probe is in a one gallon fishbowl that is also on the mat, not directly in the P. hipoliti containers). They seem to have no problem staying in the water column. I wanted to count and put equal numbers in both containers but I started doing it in the dark and then this morning I didn't have time to tinker around. So it's not very scientific, but I should get an idea of how long they can go without food before starving/dying.
Eggs hatched on the 5th night.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Wednesday, January 22, 2014 10:39 AM
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New spawn yesterday. Busy little fish! Some larvae still alive in both containers. I was able to get my hands on a small amount of 5-50uM golden pearls yesterday (a local club member had some, hooray!) so I drizzled a small amount of that in last night. This morning I swooshed around in there a bit with a turkey baster since a couple larvae seemed stuck at the surface. I put in a drop of Prime (due to the GP being in there). I also put lids on (just loosely resting) to keep dust and other gunk from flying in there. If I get a chance today I may pull one or two to look at under the scope. The best pic I could get of the larvae without the microscope 
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Friday, January 24, 2014 12:30 PM
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All dead again (in both containers) the night before day 3 (none spotted yesterday). New hatch should be tomorrow night, and I may try artificially hatching. My Gymnodinium order was supposed to be here yesterday but didn't come and will hopefully come today. Given that it has been quite cold here again lately, I am concerned that they will arrive dead :/
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Tuesday, January 28, 2014 7:29 PM
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I never managed these...good luck Shannon!
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, February 3, 2014 1:03 PM
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I estimate the size of these guys to be about 1.8 mm. I got a stage micrometer but I haven't finished tinkering with it, but based on what I did this weekend, 1.8mm is about right for a Day 1 larvae. I had a hatch Friday night. Before lights out, I pulled the clamshell with eggs and put it in a 1 gallon fish bowl that was clamped into a 10 gallon tank (that was filled and heated). The water was new ASW. I put an airstone on the eggs (well, not exactly...I tried to, but it didn't work as well as I wanted) and had it set to a low-ish aeration. The larvae started hatching nearly immediately and by the next morning I would say over 95% had hatched. So these guys do GREAT with artificial hatching. I added some gymnodinium culture water straight out of the bottle before the eggs were put in and added more the next morning. I have NO idea if there are actually gymnodinium in there...I didn't get a chance to do a microscope check that would tell me anything. I peeked quick and saw some things swimming, but that could have been tetraselmis since I was using that to feed the gymno. I also added (Friday evening) some Euterpina naups between 53-120uM. Probably much too big, but figured WTH. Saturday evening I pulled some for pics again and also added a small amount of S rotifer culture (IIRC...) but like 10 rots. Now, usually they die the night between day 2 and day 3, so I expected to see nothing but dead ones this morning, but they were still alive! New record!! (For me  ) I even siphoned the bottom and thought I would at least pull up some dead ones, but everything I bastered out of that soup was alive...so back in they went! There is also nanno, Iso and tet in the bowl. This morning I thought about adding more rots or something, but decided to wait until I got home today to try to sieve out ciliates or dinos from any of my cultures (I have a 17uM mesh that I lay over other sieves). I wouldn't have had time to size sort this morning and be sterile about it. On to pics! Day 1, AM-ish   Here is day 1 in the evening (I had a second person helping with the pics and think they turned out much better for it! Easier for me to focus and have someone else hit the "click" button):   Zoomed in a little bit (10x objective):  Way zoomed in on the yolk (what's left of it, at least)? I don't know my larval anatomy as well as I should...  Zoomed in on gut...food in there I think...just a few little pieces  I think those green spots are about 10uM, maybe slightly larger, which is right in the size range of Tetraselmis. Perhaps though, they ate something that ate the tetraselmis...not sure, and I wasn't able to get it any more in-focus than that. So...that's that for now! I won't be surprised if I get home and find them all dead, but here's to hoping they aren't! The parents laid a new nest, so I will get another chance soon enough 
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, February 3, 2014 1:49 PM
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Oh, and I wanted to add that these larvae are VERY sensitive. No matter how fast I am, every one that I pull for pictures dies. So now I know going into it that any larva pulled for pics will be sacrificed.
And the aeration in the one gallon fishbowl is minimal--just enough to keep them from getting stuck on the surface. The reason for this is two fold. First, the larvae seem sensitive and they don't appear to have too much of a problem staying in the water column even with no air. Second, gymnodinium is supposedly sensitive to aeration and handling as well.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Tuesday, February 4, 2014 8:52 AM
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Wednesday, February 5, 2014 8:58 AM
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Thursday, February 27, 2014 8:44 AM
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I pulled the PVC pipe that they are now spawning in last night for an artificial hatch. These guys are very very tolerate of artificial hatching, which is great since they don't seem to be very attracted to light. They also will hatch even with light on, which is also nice because then they could start eating right away if the light is left on. I say could start eating because I haven't really found anything they will eat yet. I ordered parvo nauplii and they arrived yesterday. I added a maybe 1/4 of my 50k nauplii order to the fish bowl...but I honestly think they may be too large for them to be interested in. Hopefully, that won't be the case. We shall see! Here is an image with the parvo naup added to a larval photo at the same magnification. Looks like it would be a tight squeeze, but some of the naups are probably smaller (hopefully, at least). The paper I read about another species in this genus said that they don't utilize as much of their mouth gape as you would think, so I am not too hopeful. 
<message edited by shannpeach on Thursday, February 27, 2014 9:01 AM>
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Saturday, March 1, 2014 2:08 AM
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Ha...I just posted in the report "have access to parvo"? Shannon, where did you order specifically only NAUPLII of Parvo?!
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Saturday, March 1, 2014 9:11 AM
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I think you can order parvo nauplii from Reed Mariculture.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Saturday, March 1, 2014 9:47 AM
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Yep! I got them from Reed. $45 or 50k nauplii 
<message edited by shannpeach on Monday, March 3, 2014 9:03 AM>
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, March 3, 2014 9:05 AM
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New record for me! I had a handful make it through the fourth night and into the fifth day, but not very many and I don't think that they look very good. I've had rotifers show up in the bowl, which is not ideal. And I also suspect that the parvo I got died...I found one alive (the one I have the pic of) but I just checked the cultures yesterday and didn't see anything moving....Hmmmm
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, March 3, 2014 8:58 PM
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The last of them died during the day today.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Thursday, April 10, 2014 8:48 AM
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Their last spawn was so impressive, I just had to post a pic:  I didn't collect the larvae from that nest, but the next nest I hope to have a few different foods to try so I plan to collect those larvae
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