Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Isochrysis galbana
Species description: motile brown algae
Culture source (link if possible
): Neptunes Gardens
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 3/12/14, restarted 7/23/14
Continuation Date: 11/1/14
Culturing Vessel Details Salinity: 1.020
Temperature: ambient room temp, approx. 70 degrees F
pH: not directly measured, but new mix is generally about 8.0-8.1
Vessel description: 1 quart plastic bottles, with plans to expand into 2 liter plastic bottles as cultures progress
Lighting description: 13w spiral cfl bulb
Lighting cycle: 16 hours light, 8 hours dark
Aeration description: rigid airline tubing with moderate flow
Methodologies Split methodology: 2/3 to 3/4 of mature culture used for feeding, remainder to inoculate new culture bottle
Culture medium description: initially used both microwave and bleach sterilization techniques. Currently only using bleach sterilization process: 1ml chlorox bleach in 1 gallon NSW, then 1quart culture bottle filled 2/3 to 3/4 full. 20 drops Guillards f/2 and 0.4ml Drs. Foster & Smith Chlorine Neutralizer added per quart bottle.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: I've found that using the bleach sterilization and dechlorination with Drs. Foster & Smith Chlorine Neutralizer works well for me and is now my sole method for culture medium prep
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<message edited by Lrood on Saturday, January 24, 2015 1:36 PM>