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Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Monday, March 31, 2014 7:54 PM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Americamysis bahia Species description: Mysid shrimp, small saltwater shrimp, bigger than brine. Culture source (link if possible ): Reed Mariculture If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 3/19/14 Continuation Date: Culturing Vessel Details Salinity: 27 ppt Temperature: 64F pH: 8 Vessel description: Two 5 gallon buckets linked with a pipe, airlift added Lighting description: ambient Lighting cycle: 10 hours dark, 14 hours day Aeration description: just what goes through the airlift, which is a lot of air, kind of a slow boil Methodologies Split methodology: I will switch the filters and the airlift as the nauplii and juveniles mature to adults, discarding or using as feed, the older parental adults. In this way, I should continue to generate nauplii and juveniles without parental predation. Culture medium description: 27ppt saltwater with live Isochrysis, Reed algae paste, Otohime A, bits of frozen mash, rotifers Cell count: unknown Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Monday, March 31, 2014 8:10 PM
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After reading Jazzybio's journal, I thought I would give these a try. I have to say, they are rather fun to keep, and watch, and I'm feeling pretty good about them. Initially, I poured them in a bucket of saltwater and as I walked the plastic bag to the trash can, I noticed that there were still some in there. I rinsed the shrimps into the goby larval tank, just to see if they would add anything. They have survived there very well, have made some juveniles, and don't seem to bother the gobie larvae or goby eggs at all. For the majority of the shrimps, I kept them in the bucket for a week or so. These shrimp are supposed to eat their young, and after a week or two, it seemed that I had no juveniles, so I decided to make a culture system that would automatically separate adults from their young. I made set of two saltbuckets, connected with 1 inch PVC, and an airlift to transfer water from one bucket to the other. Bucket A is the adult shrimp bucket and bucket b is the nauplii/juvenile bucket. I put a 1 mm needlepoint fabric filter on the bottom of the airlift to keep the adults in their place while the water and juveniles could be transferred to the nauplii side of bucket assembly. On the nauplii side of the bucket connector, I used a 100 micron filter to keep the nauplii and juveniles in their bucket. It seems to be working, as the nauplii bucket has a nice population of juveniles in it now. I do have to wash the 100 micron screen daily, as it clogs and the water levels in the buckets become uneven. These buckets are right near the sink, so it is easy to take care of this.
<message edited by KathyL on Monday, March 31, 2014 8:29 PM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Monday, March 31, 2014 8:13 PM
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I'm thinking about giving these a try soon. Can you post a pic of your set-up?
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Monday, March 31, 2014 8:33 PM
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See above, which I was populating with pix while you were posting, Tal. Here's one of the adult side: and the larval side:
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Monday, March 31, 2014 8:39 PM
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Great, thanks!
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Tuesday, April 1, 2014 9:25 AM
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I like this set-up...I think I may switch mine over to this sometime this weekend.
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Tuesday, April 1, 2014 9:47 AM
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Cool design Kathy! If you add a 3rd bucket you could do a continuous round-robin system. A senior bucket, adult bucket, and a juvenile bucket. Once you see juveniles in the 3rd bucket you remove the seniors (feed them to whatever) and then place that now empty bucket on the "bottom rung" and wait for juveniles to show up again. Now I am itching to try to keep a mysid culture even more!
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Tuesday, April 1, 2014 10:33 PM
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It seems to be working. Despite not really knowing what they eat at each stage, i've got them growing. Nauplii turning to juveniles and adults breeding and making more. I used: 2 buckets 2 uniseals, 1 inch, for bulkheads and the right size hole saw to install them, (make sure you put them level the same distance from the bottom on both buckets. I used some 1 inch PVC pipes and fittings that I had lying around. You will need 2 elbows, slip is best, and several unions for connecting the two buckets and also for making filters for both the airlift and the connector. Also some kind of glue to hold the filter material on. For the needlepoint, regular PVC cement did not work, so I used silicon. There is not a lot of pressure on this filter, so if it is not permanent it is no disaster. fine needlepoint plastic or 1mm filter I think I used 300 micron, might have been 100, polyester for the nauplii screen. One might want to use an adapter instead of a union, so you can get more surface area. A reasonably strong air pump. The smallest hobby pump won't work as an airlift. You need something stronger. Various feeds, live phytoplankton, dead phytoplankton, rotifers, tiny feed like Oto A, stuff like that--not sure what they are really eating. Apparently, you don't need a heater, or even much filtration, if you do some water changing. Reed mariculture is now selling mysids, although there may be others. I'm really having fun with this, and I hope you do too.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Tuesday, April 1, 2014 10:35 PM
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Waldend, when the juveniles are mostly adults, I'll drain their parents' bucket, refill with clean water, reverse the filters and the airlift, and keep going. Two buckets take up less real estate.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Tuesday, April 1, 2014 11:30 PM
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Ha. My brain completely blocked the thought of two buckets working. I think I am working too much and playing with fishes too little!
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Wednesday, April 2, 2014 8:51 AM
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Thanks for all the info! I actually probably have everything I'll need except for the buckets lol I just have mine in a 10 gallon tank and I feed them live nhbbs (and sometimes older ones). They pretty much get what I remove from water changes for the invert larval containers. They clear out the bbs pretty quickly, so they must be eating them. Not sure if adding rotifers would help the smaller ones eat more or not...might be worth a shot.
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Wednesday, April 2, 2014 9:34 AM
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Hey kathy, excellent work figuring out this! I'm talking to my dad to get a culture going, and he believes this could work for ghost shrimp and Arcatia tonsa. What's everyone's thoughts on this?
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Wednesday, April 2, 2014 11:25 AM
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Can you post a pic showing the actual mysis (for your report)? Also, have you harvested any yet? I have found that siphoning them to be a bit of a bugger because they are so fast. So far I have had better luck using a net with a large mesh size (it came with a 1 gallon fish bowl "set" from walmart, IIRC) that captures the larger ones, but the smaller ones can escape from.
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Wednesday, April 2, 2014 12:28 PM
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Very cool idea Kathy! You are so creative...
Don't let fear and common sense stop you! =]
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Wednesday, April 2, 2014 6:48 PM
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check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Wednesday, April 2, 2014 6:51 PM
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Mindy, it was not my original idea. but thanks.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Thursday, April 3, 2014 4:40 PM
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Originally Posted by KathyL
Mindy, it was not my original idea. but thanks. I'm going to tell it like it was.
Don't let fear and common sense stop you! =]
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Thursday, April 10, 2014 11:07 AM
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Kathy, I just want to verify/check a few things about your bucket set-up. You have the connector pipe that goes between the two buckets...do those have screen on them? And then you have your airlift that has a screen to keep the adults from entering, correct? I just started to make my new bucket set-up last night and got as far as putting the one of the pipes into one of the uniseal holes before I tuckered out for the evening lol
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Thursday, April 10, 2014 7:35 PM
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I made mine with a union between the buckets so I can disconnect them if I want to, but actually that would be really inconvenient, I think, and I've just been siphoning to clean the buckets. I have a needlepoint screen on the bottom of the airlift that throws water into the juvenile side from the adult side. The juvies go through the screen. I have a 100 or so screen on the juvie side of the connector pipe to keep the juvies in and the adults out. That said, a couple of adults got into the juvie side, and I still see juvies on the adult side. The sedimentary schmutz, however, always ends up on the juvie side.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Americamysis bahia (Mysid shrimp)
Thursday, April 10, 2014 8:23 PM
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Alright, that clarifies it for me. Thanks!
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