Reports tied to this Journal
Culture Journal, Species: Isochrysis galbana
Tuesday, July 29, 2014 11:29 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Isochrysis Galbana Species description: Motile brown microalgae Culture source (link if possible ): MBI Conference (T. Weiss) If algae, CCMP # (Optional ): [link= http://ccmp.bigelow.edu/]http://ccmp.bigelow.edu/[/link] Culture Establishment Date:7/21/2014 Continuation Date: Culturing Vessel Details Salinity: 1.020 Temperature: Ambient Room Temp (70°F-78°F) pH:Not monitored Vessel description: 1 Quart Wide-mouth canning jars. Plastic wrap cover held with rubberband around perimeter. Rigid airline poked through wrap to feed air and a small puncture for air release. Lighting description: Single T8 bulb under-cabinet lighting fixture. Lighting cycle: 18 on, 6 off Aeration description: 1-2 bubbles per second Methodologies Split methodology: Plan is to remove 75% every 7-10 days and add the remaining 25% to a new jar as an inoculation. Culture medium description: 1 litre of artificial saltwater newly mixed and aged ~ 24hrs. Sterilized in microwave until the water boils in the jar. Allowed to cool at room temp. 10 drops (~1/2ml) Micro Algae Grow added to the culture. Cell count: Not known (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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