MBI Event Reporting Form

GENERAL
Name:	Shannon Pechauer ( shannpeach ) Site: Marine Breeding Initiative (MBI)
Species:	Apocyclops panamensis (Marsh, 1913) ( Class: FC1 , Points: 5 )
Establishment Date:	04/16/2013
Culture Source:	Local Hobbyist
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=82367

Culturing Vessel Details
Temperature:	70-80 ° F
PH:	Not measured
Specific Gravity:	1.022
Vessel Description:	4L water pitchers
Lighting:	Ambient light from a southern facing window supplemented by a two-bulb strip light
Lighting Schedule:	6 AM to 9 PM

Methodologies
Feeding Tactics/Medium:	Live Tetraselmis, Isochrysis, or Chaetoceros as needed (when water clears)
Split Cycle:	Cultures are harvested when copepods are needed or when densities in the water pitchers get high (determined by eye)
Split Yield:	Not measured
Number of Cultures:	2
Number of Splits:	Numerous
Density:	Not measured
Harvest Methology:	2 to 3 L of water are poured through a 53uM sieve and fed to desired tank. Water is replaced with sterilized, aged ASW
Adjustments Made:	None

Other Details
Other Information:	Water is sterilized using 1/2 mL of bleach per 1L of water and neutralized using 1.5mL of 1M sodium thiosulfate per L of water.