MBI Event Reporting Form

GENERAL
Name:	Gina Hayward ( GinaReef ) Site: Marine Breeding Initiative (MBI)
Species:	Brachionus plicatilis ( Class: FC1 , Points: 5 )
Establishment Date:	11/07/2012
Culture Source:	Reef Nutrition (Reed Mariculture) via Incredible Aquarium in London ,Ontario
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=77144

Culturing Vessel Details
Temperature:	65*F ° F
PH:	8.4
Specific Gravity:	1.020
Vessel Description:	2 gallon ice crean tubs
Lighting:	ambient light from nanno culture
Lighting Schedule:	ambient from Nanno culture 12hr on 12hr off

Methodologies
Feeding Tactics/Medium:	2-3ml RotigrowComplete 2x/day
Split Cycle:	18 days
Split Yield:	unknown
Number of Cultures:	1
Number of Splits:	1
Density:	50+ per ml
Harvest Methology:	skimming with 53 um sieve for feeding larvae
Adjustments Made:	culture sieved (with 200um sieve)into clean culture vessel and half of the old medium discarded and top up water added at 18 days (brine shrimp contamination)

Other Details
Other Information: